Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by suppression of PI3K/Akt and MAPK pathways

被引：38

作者：

Chan, Chi-Ming ^{[1
,2
]}

Fang, Jia-You ^{[3
]}

Lin, Hsin-Huang ^{[1
]}

Yang, Chi-Yea ^{[4
]}

Hung, Chi-Feng ^{[1
]}

机构：

[1] Fu Jen Catholic Univ, Sch Med, Hsinchuang 24205, Taipei Hsien, Taiwan

[2] Cardinal Tien Hosp, Dept Ophthalmol, Taipei Hsien, Taiwan

[3] Chang Gung Univ, Grad Inst Nat Prod, Pharmaceut Lab, Tao Yuan, Taiwan

[4] Vanung Univ, Dept Biotechnol, Tao Yuan, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 388卷 / 01期

关键词：

Lycopene; Retinal pigment epithelial cell; ARPE19; PDGF; Cell migration; Cell adhesion; Cytotoxicity; Proliferative vitreoretinopathy; PI3K/Akt; MAPK; EXTRACELLULAR-MATRIX PROTEINS; HUMAN DERMAL FIBROBLASTS; PROLIFERATIVE VITREORETINOPATHY; ADJUNCTIVE TREATMENT; GROWTH-FACTOR; HUMAN HEALTH; RPE CELLS; CAROTENOIDS; ADHESION; DISEASES;

D O I：

10.1016/j.bbrc.2009.07.155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Retinal pigment epithelial (RPE) cells play a dominant role in the development of proliferative vitreoretinopathy (PVR), which is the leading cause of failure in retinal reattachment surgery. Several studies have shown that platelet-derived growth factor (PDGF) exhibits chemotaxis and proliferation effects on RPE cells in PVR. In this study, the inhibitory effect of lycopene on PDGF-BB-induced ARPE19 cell migration is examined. In electric cell-substrate impedance sensing (ECIS) and Transwell migration assays, significant suppression of PDGF-BB-induced ARPE19 cell migration by lycopene is observed. Cell viability assays show no cytotoxicity of lycopene on RPE cells. Lycopene shows no effect on ARPE19 cell adhesion and is found to inhibit PDGF-BB-induced tyrosine phosphorylation and the underlying signaling pathways of PI3K, Akt, ERK and p38 activation. However, PDGF-BB and lycopene show no effects on JNK activation. Taken together, our results demonstrate that lycopene inhibits PDGF-BB-induced ARPE19 cell migration through inhibition of PI3K/Akt, ERK and p38 activation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：172 / 176

页数：5

共 31 条

[1]

Agarwal S, 2000, CAN MED ASSOC J, V163, P739

[2] BIOLOGICAL ACTIONS OF CAROTENOIDS [J].

BENDICH, A ;

OLSON, JA .

FASEB JOURNAL, 1989, 3 (08) :1927-1932

[3] Identification and quantitation of carotenoids and their metabolites in the tissues of the human eye [J].

Bernstein, PS ;

Khachik, F ;

Carvalho, LS ;

Muir, GJ ;

Zhao, DY ;

Katz, NB .

EXPERIMENTAL EYE RESEARCH, 2001, 72 (03) :215-223

[4]

Briggs MC, 2000, INVEST OPHTH VIS SCI, V41, P3085

[5] Pathogenic mechanisms in proliferative vitreoretinopathy [J].

Campochiaro, PA .

ARCHIVES OF OPHTHALMOLOGY, 1997, 115 (02) :237-241

[6] Post traumatic proliferative vitreoretinopathy - The epidemiologic profile, onset, risk factors, and visual outcome [J].

Cardillo, JA ;

Stout, JT ;

LaBree, L ;

Azen, SP ;

Omphroy, L ;

Cui, JZ ;

Kimura, H ;

Hinton, DR ;

Ryan, SJ .

OPHTHALMOLOGY, 1997, 104 (07) :1166-1173

[7] Platelet derived growth factor and fibroblast growth factor basic levels in the vitreous of patients with vitreoretinal disorders [J].

Cassidy, L ;

Barry, P ;

Shaw, C ;

Duffy, J ;

Kennedy, S .

BRITISH JOURNAL OF OPHTHALMOLOGY, 1998, 82 (02) :181-185

[8] PROLIFERATIVE VITREORETINOPATHY - PATHOBIOLOGY, SURGICAL-MANAGEMENT, AND ADJUNCTIVE TREATMENT [J].

CHARTERIS, DG .

BRITISH JOURNAL OF OPHTHALMOLOGY, 1995, 79 (10) :953-960

[9] Proliferative vitreoretinopathy - developments in adjunctive treatment and retinal pathology [J].

Charteris, DG ;

Sethi, CS ;

Lewis, GP ;

Fisher, SK .

EYE, 2002, 16 (04) :369-374

[10] Carotenoid action on the immune response [J].

Chew, BP ;

Park, JS .

JOURNAL OF NUTRITION, 2004, 134 (01) :257S-261S

← 1 2 3 4 →