Development of a HPLC method for the estimation of celecoxib in human plasma

被引：0

作者：

Krishnaiah, YSR ^{[1
]}

Satyanarayana, V ^{[1
]}

Bhaskar, P ^{[1
]}

Raju, V ^{[1
]}

机构：

[1] Andhra Univ, Dept Pharmaceut Sci, Pharmaceut Technol Div, Visakhapatnam 530003, Andhra Pradesh, India

来源：

ASIAN JOURNAL OF CHEMISTRY | 2003年 / 15卷 / 02期

关键词：

celecoxib; human plasma; estimation; HPLC;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Varying quantity of celecoxib (25 to 1500 ng) was added to drug-free human plasma (0.5 mL) and mixed with 0.5 mL of acetonitrile. The mixture was vortexed for 5 min and centrifuged at 3,000 rpm for 10 min and the supernatant liquid was filtered. Fifty micro-litres of the resultant filtrate was injected into a reverse phase C-18 column using a mobile phase consisting of methanol and acetic acid (pH adjusted to 3.0 with dilute ammonia solution) in the ratio of 68: 32% v/v at a flow rate of 1.5 mL/min. The eluents were monitored at 254 run. The method was validated for its linearity, precision and accuracy. The present HPLC method was found to be simple, precise, specific, less time consuming and accurate for the estimation of celecoxib in human plasma.

引用

页码：949 / 952

页数：4