Transcriptome analysis of nicotine-exposed cells from the brainstem of neonate spontaneously hypertensive and Wistar Kyoto rats

被引：2
作者：
Ferrari, M. F. R. [1 ]
Reis, E. M. [2 ]
Matsumoto, J. P. P. [3 ]
Fior-Chadi, D. R. [3 ]
机构：
[1] Univ Sao Paulo, Dept Neurol, Fac Med, BR-01246903 Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01246903 Sao Paulo, Brazil
[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-01246903 Sao Paulo, Brazil
来源：
PHARMACOGENOMICS JOURNAL | 2010年 / 10卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
microarray; hypertension; nicotine; gene expression; primary cell culture; brainstem; NUCLEUS-TRACTUS-SOLITARII; ROSTRAL VENTROLATERAL MEDULLA; GENE-EXPRESSION; SYNAPSIN-III; HEART-RATE; PROTEIN; RELEASE; CORTEX; ADRENOMEDULLIN; LOCALIZATION;
D O I：
10.1038/tpj.2009.42
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
In this study, the effects of nicotine on global gene expression of cultured cells from the brainstem of spontaneously hypertensive rat (SHR) and normotensive Wistar Kyoto (WKY) rats were evaluated using whole-genome oligoarrays. We found that nicotine may act differentially on the gene expression profiles of SHR and WKY. The influence of strain was present in 321 genes that were differentially expressed in SHR as compared with WKY brainstem cells independently of the nicotine treatment. A total of 146 genes had their expression altered in both strains after nicotine exposure. Interaction between nicotine treatment and the strain was observed to affect the expression of 229 genes that participate in cellular pathways related to neurotransmitter secretion, intracellular trafficking and cell communication, and are possibly involved in the phenotypic differentiation between SHR and WKY rats, including hypertension. Further characterization of their function in hypertension development is warranted. The Pharmacogenomics Journal (2010) 10, 134-160; doi:10.1038/tpj.2009.42; published online 15 September 2009
引用
收藏
页码：134 / 160
页数：27
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