Overexpression of an AP2/ERF-type Transcription Factor CRF5 Confers Pathogen Resistance to Arabidopsis Plants

被引：25

作者：

Liang, Ying Shi ^{[2
,4
]}

Ermawati, Netty ^{[1
,2
]}

Cha, Joon-Yung ^{[1
,2
]}

Jung, Min Hee ^{[1
,2
]}

Su'udi, Mukhamad ^{[1
,2
]}

Kim, Min Gab ^{[4
]}

Ha, Sun-Hwa ^{[4
]}

Park, Chung-Gyoo ^{[2
,3
]}

Son, Daeyoung ^{[1
,3
]}

机构：

[1] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Div Appl Life Sci, Program BK21, Jinju 660701, South Korea

[3] Gyeongsang Natl Univ, Dept Appl Biol, Jinju 660701, South Korea

[4] RDA, Natl Acad Agr Sci, Suwon 441707, South Korea

来源：

JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | 2010年 / 53卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Arabidopsis; CRF5; cytolcinin; ERF-type transcription factor; pathogen resistance; DISEASE RESISTANCE; ENHANCES RESISTANCE; CYTOKININ RESPONSES; GENE; EXPRESSION; TOBACCO; MAIZE;

D O I：

10.3839/jksabc.2010.024

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The cytokinin response factor 5 (CRF5) belongs to a family of plant-specific APETALA2 (AP2)/ethylene-responsive element binding proteins (EREBPs). The novel role of Arabidopsis CRF5, previously identified as a mediator of cytokinin signaling, has been suggested to increase pathogen resistance in this study. Endogenous CRF5 transcripts are expressed in all tissues, including the seedlings, leaf, stem, flower, silique and root, and were found to be induced at 1 h after infection with the bacterial pathogen, Pseudomonas syringae pv. tomato DC3000 (Psi DC3000). The results of a yeast one-hybrid assay revealed that an acidic region of CRF5, including the C-terminal 28 amino acids, functions as a transcriptional activator. Overexpression of CRF5 in transgenic Arabidopsis increases pathogen resistance and concomitantly activates the expression of a large number of GCC-box pathogenesis-related genes. These results indicate that CRF5 may be involved in disease resistance as a transcription activator, thus providing a mechanistic link between the plant pathogen response and cytokinin signaling.

引用

页码：142 / 148

页数：7

共 22 条

[1] Cytokinins in pathogenesis and disease resistance of Pyrenophora teres-barley and Dreschslera maydis-maize interactions during early stages of infection [J].