Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization

被引：57

作者：

Moon, Seyoung ^{[1
,2
]}

Kim, Dong Jun ^{[2
]}

Kim, Kyujung ^{[1
,2
]}

Kim, Donghyun ^{[1
,2
]}

Lee, Hosub ^{[3
]}

Lee, Kangtaek ^{[1
,3
]}

Haam, Seungjoo ^{[1
,3
]}

机构：

[1] Yonsei Univ, Program Nanomed Sci & Technol, Seoul 120749, South Korea

[2] Yonsei Univ, Sch Elect & Elect Engn, Seoul 120749, South Korea

[3] Yonsei Univ, Dept Chem & Biomol Engn, Seoul 120749, South Korea

来源：

APPLIED OPTICS | 2010年 / 49卷 / 03期

关键词：

HIGHLY SENSITIVE DETECTION; OPTICAL-PROPERTIES; BIOSENSORS; FILMS; GOLD; NANOSTRUCTURES; DISPERSION; GRATINGS; SENSORS; NM;

D O I：

10.1364/AO.49.000484

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have investigated surface-enhanced plasmon resonance detection of DNA hybridization. Surface enhancement was based on the excitation of localized surface plasmon using subwavelength nanogratings, at a 300nm period, coated with 24-mer ssDNA oligonucleotide, while optical signatures of DNA were amplified at the same time by gold nanoparticles conjugated with complementary ssDNA strands. When using nanoparticles of different sizes, maximum sensitivity enhancement, of more than 18 times, was obtained with nanoparticles of 20nm diameter. This enhancement is mainly due to nanoparticle-associated signal amplification. Additional surface enhancement boosted the detection sensitivity by 57%. We have also confirmed the sensitivity enhancement to be linearly related to nanoparticle volume. (C) 2010 Optical Society of America

引用

页码：484 / 491

页数：8

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