Strategies for Applying Nonhomologous End Joining-Mediated Genome Editing in Prokaryotes

被引：11
作者：
Cui, Yali [1 ,2 ,3 ,4 ]
Dong, Huina [3 ,4 ]
Ma, Yuanyuan [1 ,2 ]
Zhang, Dawei [3 ,4 ]
机构：
[1] Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China
[2] Tianjin Univ, R&D Ctr Petrochem Technol, Tianjin 300072, Peoples R China
[3] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
[4] Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
来源：
ACS SYNTHETIC BIOLOGY | 2019年 / 8卷 / 10期
基金：
中国国家自然科学基金;
关键词：
NHEJ; genome engineering; CRISPR/Cas9; system; homologous recombination; DOUBLE-STRAND BREAKS; DNA-REPAIR; CELL-CYCLE; HOMOLOGOUS RECOMBINATION; ESCHERICHIA-COLI; BACTERIAL-DNA; KU; ATM; MECHANISM; DELETION;
D O I：
10.1021/acssynbio.9b00179
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The emergence of genome editing technology based on the CRISPR/Cas system enabled revolutionary progress in genetic engineering. Double-strand breaks (DSBs), which can be induced by the CRISPR/Cas9 system, cause serious DNA damage that can be repaired by a homologous recombination (HR) system or the nonhomologous end joining (NHEJ) pathway. However, many bacterial species have a very weak HR system. Thus, the NHEJ pathway can be used in prokaryotes. Starting with a brief introduction of the mechanism of the NHEJ pathway, this review focuses on current research and details of applications of NHEJ in eukaryotes, which forms the theoretical basis for the application of the NHEJ system in prokaryotes.
引用
收藏
页码：2194 / 2202
页数：17
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