Drug targeting Mycobacterium tuberculosis cell wall synthesis:: Development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library

被引：71

作者：

Scherman, MS

Winans, KA

Stern, RJ

Jones, V

Bertozzi, CR

McNeil, MR ^{[1
]}

机构：

[1] Colorado State Univ, Dept Microbiol, Ft Collins, CO 80523 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Mol, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Cell Biol, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 01期

关键词：

D O I：

10.1128/AAC.47.1.378-382.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A microliter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

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页码：378 / 382

页数：5

相关论文

共 9 条

[1] Enzymatic synthesis of UDP-galactofuranose and an assay for UDP-galactopyranose mutase based on high-performance liquid chromatography [J].

Lee, R ;

Monsey, D ;

Weston, A ;

Duncan, JK ;

Rithner, C ;

McNeil, M .

ANALYTICAL BIOCHEMISTRY, 1996, 242 (01) :1-7

[2] ESCHERICHIA-COLI K12 REGAINS ITS O-ANTIGEN [J].

LIU, D ;

REEVES, PR .

MICROBIOLOGY-UK, 1994, 140 :49-57

[3] Initiating a crystallographic study of UDP-galactopyranose mutase from Escherichia coli [J].

McMahon, SA ;

Leonard, GA ;

Buchanan, LV ;

Giraud, MF ;

Naismith, JH .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 :399-402

[4] Galactofuranose biosynthesis in Escherichia coli K-12: Identification and cloning of UDP-galactopyranose mutase [J].

Nassau, PM ;

Martin, SL ;

Brown, RE ;

Weston, A ;

Monsey, D ;

McNeil, MR ;

Duncan, K .

JOURNAL OF BACTERIOLOGY, 1996, 178 (04) :1047-1052

[5] Cell wall core galactofuran synthesis is essential for growth of mycobacteria [J].

Pan, F ;

Jackson, M ;

Ma, YF ;

McNeil, M .

JOURNAL OF BACTERIOLOGY, 2001, 183 (13) :3991-3998

[6] UDP-galactopyranose mutase has a novel structure and mechanism [J].

Sanders, DAR ;

Staines, AG ;

McMahon, SA ;

McNeil, MR ;

Whitfield, C ;

Naismith, JH .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (10) :858-863

[7] STRUCTURE OF THE O-ANTIGEN OF ESCHERICHIA-COLI-K-12 AND THE SEQUENCE OF ITS RFB GENE-CLUSTER [J].

STEVENSON, G ;

NEAL, B ;

LIU, D ;

HOBBS, M ;

PACKER, NH ;

BATLEY, M ;

REDMOND, JW ;

LINDQUIST, L ;

REEVES, P .

JOURNAL OF BACTERIOLOGY, 1994, 176 (13) :4144-4156

[8] Biosynthetic origin of mycobacterial cell wall galactofuranosyl residues [J].

Weston, A ;

Stern, RJ ;

Lee, RE ;

Nassau, PM ;

Monsey, D ;

Martin, SL ;

Scherman, MS ;

Besra, GS ;

Duncan, K ;

McNeil, MR .

TUBERCLE AND LUNG DISEASE, 1998, 78 (02) :123-131

[9] An inhibitor of the human UDP-GlcNAc 4-epimerase identified from a uridine-based library:: A strategy to inhibit O-linked glycosylation [J].

Winans, KA ;

Bertozzi, CR .

CHEMISTRY & BIOLOGY, 2002, 9 (01) :113-129