Screening for Acetylcholinesterase Inhibitory Activity in Plant Extracts from Argentina

被引：40

作者：

Carpinella, Maria C. ^{[1
]}

Andrione, Diego G.

Ruiz, Gustavo ^{[2
]}

Palacios, Sara M.

机构：

[1] Univ Catolica Cordoba, Fac Ciencias Quim, Sch Chem, Fine Chem & Nat Prod Lab, RA-5000 Cordoba, Argentina

[2] Univ Catolica Cordoba, Sch Agr Sci, Herbarium Marcelino Sayago, RA-5000 Cordoba, Argentina

来源：

PHYTOTHERAPY RESEARCH | 2010年 / 24卷 / 02期

关键词：

acetylcholinesterase inhibitors; plant extracts; TLC assay; Ellman's method; THIN-LAYER-CHROMATOGRAPHY; FLAVONOIDS; SCOPOLETIN; PRODUCTS; FRUITS;

D O I：

10.1002/ptr.2923

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Plants are a potential source of bioactive compounds and offer a promising strategy for the treatment of neurological disorders such as Alzheimer's disease. The inhibitory effect of 73 native and naturalized plants collected from the central region of Argentina oil acetylcholinesterase (AChE) was tested using microplate and TLC assays after solvent fractionation of complete ethanol extracts obtained from the plants. Organic fractions obtained from extracts of Achyrocline tomentosa (Asteraceae), Eupatorium viscidum (Asteraceae), Ruprechtia apetala (Polygonaceae) Trichocline reptans (Asteraceae) and Zanthoxylum coco (Rutaceae) presented strong inhibition of AChE (higher than 80%) at 1 mg/mL, with R. apetala and T. reptans being the most potent, showing complete inhibition of the enzyme. Their IC(50) values were 0.0779 and 0.1118 mg/mL, respectively. Aqueous fractions did not show any inhibitory activity on the enzyme. These results suggest that the most effective extracts deserve further investigation with the aim of obtaining new molecules for the treatment of neurodegenerative disorders. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：259 / 263

页数：5

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