Alzheimer Amyloid Peptide Aβ42 Regulates Gene Expression of Transcription and Growth Factors

被引:49
作者
Barucker, Christian [1 ,2 ]
Sommer, Anette [3 ]
Beckmann, Georg [3 ]
Eravci, Murat [1 ]
Harmeier, Anja [1 ]
Schipke, Carola G. [4 ]
Brockschnieder, Damian [3 ]
Dyrks, Thomas [3 ]
Althoff, Veit [1 ]
Fraser, Paul E. [5 ,6 ]
Hazrati, Lili-Naz [7 ]
St George-Hyslop, Peter [5 ,6 ]
Breitner, John C. S. [8 ,9 ]
Peters, Oliver [4 ]
Multhaup, Gerhard [1 ,2 ]
机构
[1] Free Univ Berlin, Inst Chem & Biochem, Berlin, Germany
[2] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada
[3] Bayer Pharma AG, Global Drug Discovery, Berlin, Germany
[4] Charite, Dept Psychiat & Psychotherapy, D-13353 Berlin, Germany
[5] Univ Toronto, Dept Med, Tanz Ctr Res Neurodegenerat Dis, Toronto, ON, Canada
[6] Univ Toronto, Dept Med Biophys, Tanz Ctr Res Neurodegenerat Dis, Toronto, ON, Canada
[7] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[8] Douglas Mental Hlth Univ Inst, Verdun, PQ, Canada
[9] McGill Univ, Fac Med, Dept Psychiat, Montreal, PQ H3G 1Y6, Canada
关键词
Alzheimer's disease; amyloid-beta; CSF proteomics; gene regulation; ID1-3; IGFBP; immunohistochemistry; LMO4; transcription factors; CEREBROSPINAL-FLUID; PRECURSOR PROTEIN; TRANSGENIC MICE; BETA OLIGOMERS; FACTOR-I; COGNITIVE DEFICITS; HYPOTHETICAL MODEL; DYNAMIC BIOMARKERS; BINDING PROTEIN-3; INSULIN-RECEPTORS;
D O I
10.3233/JAD-141902
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The pathogenesis of Alzheimer's disease (AD) is characterized by the aggregation of amyloid-beta (A beta) peptides leading to deposition of senile plaques and a progressive decline of cognitive functions, which currently remains the main criterion for its diagnosis. Robust biomarkers for AD do not yet exist, although changes in the cerebrospinal fluid levels of tau and A beta represent promising candidates in addition to brain imaging and genetic risk profiling. Although concentrations of soluble A beta(42) correlate with symptoms of AD, less is known about the biological activities of A beta peptides which are generated from the amyloid-beta protein precursor. An unbiased DNA microarray study showed that A beta(42), at sub-lethal concentrations, specifically increases expression of several genes in neuroblastoma cells, notably the insulin-like growth factor binding proteins 3 and 5 (IGFBP3/5), the transcription regulator inhibitor of DNA binding, and the transcription factor Lim only domain protein 4. Using qRT-PCR, we confirmed that mRNA levels of the identified candidate genes were exclusively increased by the potentially neurotoxic A beta(42) wild-type peptide, as both the less toxic A beta(40) and a non-toxic substitution peptide A beta(42) G33A did not affect mRNA levels. In vivo immunohistochemistry revealed a corresponding increase in both hippocampal and cortical IGFBP5 expression in an AD mouse model. Proteomic analyses of human AD cerebrospinal fluid displayed increased in vivo concentrations of IGFBPs. IGFBPs and transcription factors, as identified here, are modulated by soluble A beta(42) and may represent useful early biomarkers.
引用
收藏
页码:613 / 624
页数:12
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