Solid-phase peptide synthesis in a microfluidic device

被引：0

作者：

Maruyama, T

Uchida, J

Ohkawa, T

Kubota, F

Kamiya, N

Goto, M

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8128581, Japan

[2] Tosoh Corp, Tokyo Res Labs, Ayase, Kanagawa 2521123, Japan

来源：

KAGAKU KOGAKU RONBUNSHU | 2004年 / 30卷 / 02期

关键词：

microreactor; oligopeptide; peptide synthesis; solid-phase synthesis;

D O I：

10.1252/kakoronbunshu.30.180

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 mum, depth 160 mum) with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

引用

页码：180 / 182

页数：3

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