Characterisation of an isolate of Narcissus degeneration virus from Chinese narcissus (Narcissus tazetta var. chinensis)

被引:8
作者
Chen, J. [1 ]
Shi, Y. -H.
Adams, M. J.
Zheng, H. -Y.
Qin, B. -X.
Chen, J. -P. [1 ]
机构
[1] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310027, Peoples R China
[3] Rothamsted Res, Plant Pathogen Interact Div, Wheat Pathogenesis Programme, Harpenden, Herts, England
[4] Guangxi Acad Agr Sci, Dept Plant Protect, Nanning, Peoples R China
关键词
Soybean Mosaic Virus; Zucchini Yellow Mosaic Virus; Bean Yellow Mosaic Virus; Onion Yellow Dwarf Virus; Lettuce Mosaic Virus;
D O I
10.1007/s00705-006-0841-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with eighteen other potyviruses. The complete nucleotide sequence ( 9816 nt) had the typical genome organisation for a member of the genus Potyvirus. Sequence comparisons and phylogenetic analysis showed that the Chinese virus was different from all previously sequenced potyviruses but distantly related to onion yellow dwarf and shallot yellow stripe viruses.
引用
收藏
页码:441 / 448
页数:8
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