Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells

被引：5
作者：
Kim, Chang Min [1 ,2 ]
Jang, Tae-ho [1 ,2 ]
Park, Hyun Ho [1 ,2 ]
机构：
[1] Yeungnam Univ, Sch Chem & Biochem, Gyongsan, South Korea
[2] Yeungnam Univ, Grad Sch Biochem, Gyongsan, South Korea
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2016年 / 179卷 / 04期
关键词：
Embryonic stemcell; Pluripotency factor; TFCP2L1; SAM domain; CP2; domain; SELF-RENEWAL; PATHWAYS; NETWORK; POLYMER; CORE; CP2;
D O I：
10.1007/s12010-016-2021-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
TFCP2L1 is a transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells by forming a complex transcriptional network with other transcription factors (OCT4, SOX2, and NANOG). TFCP2L1 contains two distinct domains, the CP2-like domain at the N-terminus and the SAM-like domain at the C-terminus. In this study, we found that TFCP2L1 is hexamerized in solution via the C-terminal SAM-like domain. We also found that homo-oligomerization of SAM-like domain is dependent on the concentration of the proteins. Finally, we found that TFCP2L1 binds directly to DNA via the N-terminal CP2-like domain.
引用
收藏
页码：650 / 658
页数：9
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