Mexican stingless bee honey was already used in ancient civilizations for food and medicinal purposes. In this study, certain physical and chemical parameters of honey [liquid and powder (spray-dried)] produced by the stingless bee Scaptotrigona pectoralis from Yucatan, Mexico, were determined. Measured parameters in liquid honey were as follows: color (85.0 mm Pfund), moisture (25%), pH (3.6), acidity (116.0 meq kg(-1)), ash (0.25%), hydroxymethylfurfural (43.0 mg kg(-1)), invertase activity (22.0 IN) and reducing sugars [38.0 g (10 kg(-1))]. Of nineteen volatiles compounds positively identified, p-anisaldehyde was found as the major compound. The antioxidant activity was evaluated by using the methods ABTS(center dot+) [37.0 mu mol ET (10 kg(-1))] and DPPH [3.50 mu mol ET 10 kg(-1)]. Polyphenols content was 23.0 mg EAG 10 kg(-1). Honey antioxidant activities were by ABTS(center dot+) [37.0 mu mol ET (10 kg(-1))] and DPPH [3.5 mu mol ET (10 kg(-1))]. Polyphenols content was 23.0 mg EAG (10 kg(-1)). On the other hand, the spray-dried honey of S. pectoralis, presented high hygroscopicity [22.0 g (10 kg(-1))], good solubility (265 s), low density (0.34 g ml(-1)) and good volatiles retention (77%). The antioxidant activity of the honey powder was higher [51 mu mol ET (10 kg(-1))] (ABTS(center dot+) method) than in the liquid honey. The results show new information about the composition of the liquid honey and honey powder, which helps to assess their qualities. Further, the spray-drying process could be an alternative to preserve and even improve the antioxidant activity of S. pectoralis honey probably due to enrichment of antioxidant compounds in the powder.