Neuroprotective effects of glucomoringin-isothiocyanate against H2O2-Induced cytotoxicity in neuroblastoma (SH-SY5Y) cells

被引：22

作者：

Jaafaru, Mohammed Sani ^{[1
,2
]}

Nordin, Norshariza ^{[3
]}

Rosli, Rozita ^{[3
,4
]}

Shaari, Khozirah ^{[5
,6
]}

Bako, Hauwa'u Yakubu ^{[2
]}

Saad, Norazalina ^{[4
]}

Noor, Noramaliza Mohd ^{[7
]}

Razis, Ahmad Faizal Abdull ^{[1
,8
,9
]}

机构：

[1] Univ Putra Malaysia, Inst Biosci, Lab Mol Biomed, Upm Serdang 43400, Selangor, Malaysia

[2] Kaduna State Univ, Dept Biochem, Main Campus,PMB 2339, Kaduna, Nigeria

[3] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Biomed Sci, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Inst Biosci, UPM MAKNA Canc Res Lab, Upm Serdang 43400, Selangor, Malaysia

[5] Univ Putra Malaysia, Inst Biosci, Lab Nat Prod, Upm Serdang 43400, Selangor, Malaysia

[6] Univ Putra Malaysia, Fac Sci, Dept Chem, Upm Serdang 43400, Selangor, Malaysia

[7] Univ Putra Malaysia, Dept Imaging, Fac Med & Hlth Sci, Upm Serdang 43400, Selangor, Malaysia

[8] Univ Putra Malaysia, Dept Food Sci, Fac Food Sci & Technol, Upm Serdang 43400, Selangor, Malaysia

[9] Univ Putra Malaysia, Lab Food Safety & Food Integr, Inst Trop Agr & Food Secur, Upm Serdang 43400, Selangor, Malaysia

来源：

NEUROTOXICOLOGY | 2019年 / 75卷

关键词：

Glucomoringin isothiocyanate; Neurodegeneration; Neuroprotection; Signalling pathways; Neuroblastoma; SH-SY5Y differentiation; NF-KAPPA-B; ALZHEIMERS-DISEASE; 4(ALPHA-L-RHAMNOSYLOXY)-BENZYL ISOTHIOCYANATE; NEURODEGENERATIVE DISEASES; NEURONAL APOPTOSIS; PROTEIN ABUNDANCE; OXIDATIVE STRESS; MOUSE MODEL; ACTIVATION; ROLES;

D O I：

10.1016/j.neuro.2019.09.008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neurodegenerative diseases (NDDs) are pathological conditions characterised by progressive damage of neuronal cells leading to eventual loss of structure and function of the cells. Due to implication of multi-systemic complexities of signalling pathways in NDDs, the causes and preventive mechanisms are not clearly delineated. The study was designed to investigate the potential signalling pathways involved in neuroprotective activities of purely isolated glucomoringin isothiocyanate (GMG-ITC) against H2O2-induced cytotoxicity in neuroblastoma (SH-SY5Y) cells. GMG-ITC was isolated from Moringa oleifera seeds, and confirmed with NMR and LC-MS based methods. Gene expression analysis of phase II detoxifying markers revealed significant increase in the expression of all the genes involved, due to GMG-ITC pre-treatment. GMG-ITC also caused significant decreased in the expression of NF-kB, BACE1, APP and increased the expressions of IkB and MAPT tau genes in the differentiated cells as confirmed by multiplex genetic system analysis. The effect was reflected on the expressed proteins in the differentiated cells, where GMG-ITC caused increased in expression level of Nrf2, SOD-1, NQO1, p52 and c-Rel of nuclear factor erythroid factor 2 (Nrf2) and nuclear factor kappa-B (NF-kB) pathways respectively. The findings revealed the potential of GMG-ITC to abrogate oxidative stress-induced neurodegeneration through Nrf2 and NF-kB signalling pathways.

引用

页码：89 / 104

页数：16

共 74 条

[1] Moringa oleifera as an Anti-Cancer Agent against Breast and Colorectal Cancer Cell Lines [J].