Highly selective staining and quantification of intracellular lipid droplets with a compact push-pull fluorophore based on benzothiadiazole

被引：21
|
作者：
Suarez, S. Israel [1 ]
Warner, Caroline C. [2 ]
Brown-Harding, Heather [3 ]
Thooft, Andrea M. [2 ]
VanVeller, Brett [2 ]
Lukesh, John C. [1 ]
机构：
[1] Wake Forest Univ, Dept Chem, Winston Salem, NC 27101 USA
[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA
[3] Wake Forest Univ, Dept Biol, Winston Salem, NC 27101 USA
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2020年 / 18卷 / 03期
基金：
美国国家科学基金会;
关键词：
NILE RED; FLUORESCENT BENZOTHIADIAZOLE; PROBES; CELLS;
D O I：
10.1039/c9ob02486g
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A robust lipophilic dye, based on the structures of the benzothiadiazole heterocycle, was shown to be a potent fluorescent stain for the selective imaging of lipid droplets (LDs) within both live and fixed human cells. Its small molecular framework, large Stokes shift, and vastly improved photostability over that of the current status quo, Nile Red, highlight its tremendous potential as a versatile chemical tool for facilitating LD imaging and research.
引用
收藏
页码：495 / 499
页数：5
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