Insulin regulates the expression of the enhancer of split- and hairy-related protein-2 gene via different pathways in 3T3-L1 adipocytes and L6 myotubes

被引：10

作者：

Kawata, H

Yamada, K ^{[1
]}

Matsuura, K

Shou, Z

Miyamoto, K

机构：

[1] Univ Fukui, Fac Med Sci, Dept Biochem, Fukui 9101193, Japan

[2] JST, CREST, Fukui 9101193, Japan

来源：

HORMONE AND METABOLIC RESEARCH | 2004年 / 36卷 / 08期

关键词：

insulin; signaling pathway; gene expression; phosphoinositide; 3-kinase; mitogen-activated protein kinase;

D O I：

10.1055/s-2004-825754

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We investigated the effect of insulin on the expression of the enhancer of split- and hairy-related protein-2 gene in 3T3-L1 adipocytes and L6 myotubes. The level of enhancer of split- and hairy-related protein-2 mRNA was increased by insulin in both cells. While both wortmannin and LY294002 blocked the increase in 3T3-L1 adipocytes, and only PD98059 was effective in L6 myotubes. Although the increase by insulin in these cells was inhibited by treatment with actinomycin D, this was enhanced by treatment with cycloheximide. Furthermore, cyclic AMP increased the level of enhancer of split- and hairy-related protein-2 mRNA in both cells in an additive manner. Thus, we conclude that insulin and cyclic AMP induce the expression of the enhancer of split- and hairy-related protein-2 gene in both 3T3-L1 adipocytes and L6 myotubes, and that the gene expression enhanced by insulin is regulated by the cell type-specific pathway. The former requires a phosphoinositide 3-kinase pathway and the latter a mitogen-activated protein kinase pathway.

引用

页码：526 / 530

页数：5

共 23 条

[1] Assessment of the roles of mitogen-activated protein kinase, phosphatidylinositol 3-kinase, protein kinase B, and protein kinase C in insulin inhibition of cAMP-induced phosphoenolpyruvate carboxykinase gene transcription [J].