A graphene nanoplatelet-polydopamine molecularly imprinted biosensor for Ultratrace creatinine detection

被引：42
作者：
Li, Yixuan [1 ]
Luo, Liuxiong [2 ]
Nie, Mengyan [1 ]
Davenport, Andrew [3 ]
Li, Ying [4 ]
Li, Bing [1 ]
Choy, Kwang-Leong [1 ]
机构：
[1] UCL, Inst Mat Discovery, Fac Math & Phys Sci, Dept Chem, London WC1E 7JE, England
[2] Cent South Univ, Sch Mat Sci & Engn, Changsha 410083, Peoples R China
[3] UCL, Royal Free Hosp, UCL Dept Renal Med, Rowland Hill St, London NW3 2PF, England
[4] UCL Inst Neurol, Dept Brain Repair & Rehabil, Queen Sq, London WC1N 3BG, England
来源：
BIOSENSORS & BIOELECTRONICS | 2022年 / 216卷
关键词：
Ultratrace creatinine detection; Molecularly imprinted polymer; Electrochemical biosensor; Graphene nanoplatelet; Polydopamine; Kidney disease; POLYMER; SENSOR; RECOGNITION;
D O I：
10.1016/j.bios.2022.114638
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Accurate and reliable analysis of creatinine is clinically important for the early detection and monitoring of patients with kidney disease. We report a novel graphene nanoplatelet (GNP)/polydopamine (PDA)-molecularly imprinted polymer (MIP) biosensor for the ultra-trace detection of creatinine in a range of body fluids. Dopamine hydrochloride (DA) monomers were polymerized using a simple one-pot method to form a thin PDA-MIP layer on the surface of GNP with high density of creatinine recognition sites. This novel surface-MIP strategy resulted in a record low limit-of-detection (LOD) of 2 x 10(-2) pg/ml with a wide dynamic detection range between 1 x 10(-1)-1 x 10(9) pg/ml. The practical application of this GNP/PDA-MIP biosensor has been tested by measuring creatinine in human serum, urine, and peritoneal dialysis (PD) fluids. The average recovery rate was 93.7-109.2% with relative standard deviation (RSD) below 4.1% compared to measurements made using standard clinical laboratory methods. Our GNP/PDA-MIP biosensor holds high promise for further development as a rapid, accurate, point-of-care diagnostic platform for detecting and monitoring patients with kidney disease.
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页数：8
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