A graphene nanoplatelet-polydopamine molecularly imprinted biosensor for Ultratrace creatinine detection

被引：55
作者：
Li, Yixuan [1 ]
Luo, Liuxiong [2 ]
Nie, Mengyan [1 ]
Davenport, Andrew [3 ]
Li, Ying [4 ]
Li, Bing [1 ]
Choy, Kwang-Leong [1 ]
机构：
[1] UCL, Inst Mat Discovery, Fac Math & Phys Sci, Dept Chem, London WC1E 7JE, England
[2] Cent South Univ, Sch Mat Sci & Engn, Changsha 410083, Peoples R China
[3] UCL, Royal Free Hosp, UCL Dept Renal Med, Rowland Hill St, London NW3 2PF, England
[4] UCL Inst Neurol, Dept Brain Repair & Rehabil, Queen Sq, London WC1N 3BG, England
来源：
BIOSENSORS & BIOELECTRONICS | 2022年 / 216卷
关键词：
Ultratrace creatinine detection; Molecularly imprinted polymer; Electrochemical biosensor; Graphene nanoplatelet; Polydopamine; Kidney disease; POLYMER; SENSOR; RECOGNITION;
D O I：
10.1016/j.bios.2022.114638
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Accurate and reliable analysis of creatinine is clinically important for the early detection and monitoring of patients with kidney disease. We report a novel graphene nanoplatelet (GNP)/polydopamine (PDA)-molecularly imprinted polymer (MIP) biosensor for the ultra-trace detection of creatinine in a range of body fluids. Dopamine hydrochloride (DA) monomers were polymerized using a simple one-pot method to form a thin PDA-MIP layer on the surface of GNP with high density of creatinine recognition sites. This novel surface-MIP strategy resulted in a record low limit-of-detection (LOD) of 2 x 10(-2) pg/ml with a wide dynamic detection range between 1 x 10(-1)-1 x 10(9) pg/ml. The practical application of this GNP/PDA-MIP biosensor has been tested by measuring creatinine in human serum, urine, and peritoneal dialysis (PD) fluids. The average recovery rate was 93.7-109.2% with relative standard deviation (RSD) below 4.1% compared to measurements made using standard clinical laboratory methods. Our GNP/PDA-MIP biosensor holds high promise for further development as a rapid, accurate, point-of-care diagnostic platform for detecting and monitoring patients with kidney disease.
引用
收藏
页数：8
相关论文
共 30 条
[11]   Global Prevalence of Chronic Kidney Disease - A Systematic Review and Meta-Analysis [J].
Hill, Nathan R. ;
Fatoba, Samuel T. ;
Oke, Jason L. ;
Hirst, Jennifer A. ;
O'Callaghan, Christopher A. ;
Lasserson, Daniel S. ;
Hobbs, F. D. Richard .
PLOS ONE, 2016, 11 (07)
[12]  
Kalantar-Zadeh K, LANCET, V379, P165
[13]   Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection [J].
Li, Bing ;
Pan, Genhua ;
Avent, Neil D. ;
Lowry, Roy B. ;
Madgett, Tracey E. ;
Waines, Paul L. .
BIOSENSORS & BIOELECTRONICS, 2015, 72 :313-319
[14]   Porous Carbon Networks Derived From Graphitic Carbon Nitride for Efficient Oxygen Reduction Reaction [J].
Li, Chenxia ;
Li, Xuesong ;
Sun, Xiaojuan ;
Zhang, Xueyu ;
Duan, Lianfeng ;
Yang, Xijia ;
Wang, Liying ;
Lu, Wei .
NANOSCALE RESEARCH LETTERS, 2019, 14 (1)
[15]   A Sensitive and Renewable Chlortoluron Molecularly Imprinted Polymer Sensor Based on the Gate-Controlled Catalytic Electrooxidation of H2O2 on Magnetic Nano-NiO [J].
Li, Xue ;
Zhang, Lianming ;
Wei, Xiaoping ;
Li, Jianping .
ELECTROANALYSIS, 2013, 25 (05) :1286-1293
[16]   Enhanced electrochemical biosensing of alpha-fetoprotein based on three-dimensional macroporous conducting polymer polyaniline [J].
Liu, Shuo ;
Ma, Yihui ;
Cui, Min ;
Luo, Xiliang .
SENSORS AND ACTUATORS B-CHEMICAL, 2018, 255 :2568-2574
[17]   Molecularly imprinted polymers on graphene oxide surface for EIS sensing of testosterone [J].
Liu, Weilu ;
Ma, Yukai ;
Sun, Guoxiang ;
Wang, Shupiao ;
Deng, Jiayin ;
Wei, Hong .
BIOSENSORS & BIOELECTRONICS, 2017, 92 :305-312
[18]  
Mahdi M.A., 2022, JIJOHE, V47
[19]   A review on creatinine measurement techniques [J].
Mohabbati-Kalejahi, Elham ;
Azimirad, Vahid ;
Bahrami, Manouchehr ;
Ganbari, Ahmad .
TALANTA, 2012, 97 :1-8
[20]   Trace-level sensing of creatine in real sample using a zwitterionic molecularly imprinted polymer brush grafted to sol-gel modified graphite electrode [J].
Patel, Amit Kumar ;
Sharma, Piyush Sindhu ;
Prasad, Bhim Bali .
THIN SOLID FILMS, 2010, 518 (10) :2847-2853
123