Mapping human cell phenotypes to genotypes with single-cell genomics

被引：64

作者：

Camp, J. Gray ^{[1
]}

Platt, Randall ^{[2
]}

Treutlein, Barbara ^{[2
]}

机构：

[1] Inst Mol & Clin Ophthalmol Basel, Basel, Switzerland

[2] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Basel, Switzerland

来源：

SCIENCE | 2019年 / 365卷 / 6460期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

HUMAN STEM-CELL; SELF-ORGANIZATION; CRISPR; ORGANOIDS; SCREENS; SEQ; DNA; TRANSCRIPTION; TECHNOLOGIES; GENERATION;

D O I：

10.1126/science.aax6648

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cumulative activity of all of the body's cells, with their myriad interactions, life histories. and environmental experiences, gives rise to a condition that is distinctly human and specific to each individual. It is an enduring goal to catalog our human cell types, to understand how they develop, how they vary between individuals, and how they fail in disease. Single-cell genomics has revolutionized this endeavor because sequencing-based methods provide a means to quantitatively annotate cell states on the basis of high-information content and high-throughput measurements. Together with advances in stem cell biology and gene editing, we are in the midst of a fascinating journey to understand the cellular phenotypes that compose human bodies and how the human genome is used to build and maintain each cell. Here, we will review recent advances into how single-cell genomics is being used to develop personalized phenotyping strategies that cross subcellular, cellular, and tissue scales to link our genome to our cumulative cellular phenotypes.

引用

页码：1401 / +

页数：5

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