siRNAs can function as miRNAs

被引：934
作者：
Doench, JG
Petersen, CP
Sharp, PA [1 ]
机构：
[1] MIT, Ctr Canc Res, Dept Biol, Cambridge, MA 02139 USA
[2] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
来源：
GENES & DEVELOPMENT | 2003年 / 17卷 / 04期
关键词：
siRNA; miRNA; RNAi; translational repression; 3 ' UTR;
D O I：
10.1101/gad.1064703
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA (siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3' UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs.
引用
收藏
页码：438 / 442
页数：5
相关论文
共 32 条
[1]   Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D-melanogaster germline [J].
Aravin, AA ;
Naumova, NM ;
Tulin, AV ;
Vagin, VV ;
Rozovsky, YM ;
Gvozdev, VA .
CURRENT BIOLOGY, 2001, 11 (13) :1017-1027
[2]   Control of developmental timing by small temporal RNAs: a paradigm for RNA-mediated regulation of gene expression [J].
Banerjee, D ;
Slack, F .
BIOESSAYS, 2002, 24 (02) :119-129
[3]   RNA silencing - Diced defence [J].
Baulcombe, D .
NATURE, 2001, 409 (6818) :295-296
[4]   Role for a bidentate ribonuclease in the initiation step of RNA interference [J].
Bernstein, E ;
Caudy, AA ;
Hammond, SM ;
Hannon, GJ .
NATURE, 2001, 409 (6818) :363-366
[5]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[6]   Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate [J].
Elbashir, SM ;
Martinez, J ;
Patkaniowska, A ;
Lendeckel, W ;
Tuschl, T .
EMBO JOURNAL, 2001, 20 (23) :6877-6888
[7]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[8]   Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C-elegans developmental timing [J].
Grishok, A ;
Pasquinelli, AE ;
Conte, D ;
Li, N ;
Parrish, S ;
Ha, I ;
Baillie, DL ;
Fire, A ;
Ruvkun, G ;
Mello, CC .
CELL, 2001, 106 (01) :23-34
[9]   A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation [J].
Ha, I ;
Wightman, B ;
Ruvkun, G .
GENES & DEVELOPMENT, 1996, 10 (23) :3041-3050
[10]   A species of small antisense RNA in posttranscriptional gene silencing in plants [J].
Hamilton, AJ ;
Baulcombe, DC .
SCIENCE, 1999, 286 (5441) :950-952
1234