Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10

被引:20
作者
Abdel-Rahman, Mohamed Ali [1 ,2 ]
Hassan, Saad El-Din [1 ]
Alrefaey, Hassan M. A. [1 ]
Elsakhawy, Tamer [3 ]
机构
[1] Al Azhar Univ, Dept Bot & Microbiol, Fac Sci, Cairo 11884, Egypt
[2] Al Azhar Univ, Al Azhar Ctr Fermentat Biotechnol & Appl Microbio, Cairo 11884, Egypt
[3] Agr Res Ctr ARC, Soil Water & Environm Res Inst SWERI, Agr Microbiol Dept, Sakha Agr Res Stn, Giza 11651, Egypt
来源
FERMENTATION-BASEL | 2021年 / 7卷 / 01期
关键词
lactic acid; designed biomass; lignocellulose-derived sugars; mixed sugars; B; coagulans; CCR; homofermentation; REPEATED BATCH FERMENTATION; EMPTY FRUIT BUNCH; ETHANOL-PRODUCTION; BUTANOL PRODUCTION; RICE STRAW; L-LACTATE; HYDROLYSATE; ACETONE; LIGNOCELLULOSE; BACTERIA;
D O I
10.3390/fermentation7010028
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lignocellulosic and algal biomass are promising substrates for lactic acid (LA) production. However, lack of xylose utilization and/or sequential utilization of mixed-sugars (carbon catabolite repression, CCR) from biomass hydrolysates by most microorganisms limits achievable titers, yields, and productivities for economical industry-scale production. This study aimed to design lignocellulose-derived substrates for efficient LA production by a thermophilic, xylose-utilizing, and inhibitor-resistant Bacillus coagulans Azu-10. This strain produced 102.2 g/L of LA from 104 g/L xylose at a yield of 1.0 g/g and productivity of 3.18 g/L/h. The CCR effect and LA production were investigated using different mixtures of glucose (G), cellobiose (C), and/or xylose (X). Strain Azu-10 has efficiently co-utilized GX and CX mixture without CCR; however, total substrate concentration (>75 g/L) was the only limiting factor. The strain completely consumed GX and CX mixture and homoferemnatively produced LA up to 76.9 g/L. On the other hand, fermentation with GC mixture exhibited obvious CCR where both glucose concentration (>25 g/L) and total sugar concentration (>50 g/L) were the limiting factors. A maximum LA production of 50.3 g/L was produced from GC mixture with a yield of 0.93 g/g and productivity of 2.09 g/L/h. Batch fermentation of GCX mixture achieved a maximum LA concentration of 62.7 g/L at LA yield of 0.962 g/g and productivity of 1.3 g/L/h. Fermentation of GX and CX mixture was the best biomass for LA production. Fed-batch fermentation with GX mixture achieved LA production of 83.6 g/L at a yield of 0.895 g/g and productivity of 1.39 g/L/h.
引用
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页数:17
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