Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces

被引：23
作者：
Ferreira, Alessandra Einsfeld [1 ]
Canal, Natalia [1 ]
Morales, Daiana [1 ]
Fuentefria, Daiane Bopp [1 ]
Corcao, Gertrudes [1 ]
机构：
[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Microbiol, BR-90050170 Porto Alegre, RS, Brazil
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2007年 / 50卷 / 02期
关键词：
Enterococcus mundtii; enterocins; antimicrobial activity; biopreservative;
D O I：
10.1590/S1516-89132007000200010
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii. Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121 degrees C for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU/mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.
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页码：249 / 258
页数：10
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