Femtosecond laser bone ablation with a high repetition rate fiber laser source

被引：40
作者：
Mortensen, Luke J. [1 ,2 ,3 ]
Alt, Clemens [2 ,3 ]
Turcotte, Raphael [2 ,3 ,4 ]
Masek, Marissa [2 ,3 ]
Liu, Tzu-Ming [2 ,3 ,5 ]
Cote, Daniel C. [6 ]
Xu, Chris [7 ]
Intini, Giuseppe [1 ,8 ]
Lin, Charles P. [2 ,3 ,8 ]
机构：
[1] Harvard Univ, Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 USA
[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Adv Microscopy Program,Ctr Syst Biol, Boston, MA USA
[3] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA USA
[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
[5] Natl Taiwan Univ, Inst Biomed Engn, Taipei 10764, Taiwan
[6] Univ Laval, Ctr Rech Univ Laval Robert Giffard, Quebec City, PQ G1J 2G3, Canada
[7] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY USA
[8] Harvard Stem Cell Inst, Cambridge, MA USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 01期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
STEM-CELLS; MICROSCOPY; PULSES; OSTEOTOMY; DAMAGE; TISSUES; REGENERATION; FLUORESCENCE; DIELECTRICS; BREAKDOWN;
D O I：
10.1364/BOE.6.000032
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Femtosecond laser pulses can be used to perform very precise cutting of material, including biological samples from subcellular organelles to large areas of bone, through plasma-mediated ablation. The use of a kilohertz regenerative amplifier is usually needed to obtain the pulse energy required for ablation. This work investigates a 5 megahertz compact fiber laser for near-video rate imaging and ablation in bone. After optimization of ablation efficiency and reduction in autofluorescence, the system is demonstrated for the in vivo study of bone regeneration. Image-guided creation of a bone defect and longitudinal evaluation of cellular injury response in the defect provides insight into the bone regeneration process. (C) 2014 Optical Society of America
引用
收藏
页码：32 / 42
页数：11
相关论文
共 50 条
[1]   Femtosecond laser ablation of dentin [J].
Alves, S. ;
Oliveira, V. ;
Vilar, R. .
JOURNAL OF PHYSICS D-APPLIED PHYSICS, 2012, 45 (24)
[2]  
An R, 2013, J BIOMED OPT, V18, DOI 10.1117/1.JBO.18.7.070504
[3]   Precise ablation of dental hard tissues with ultra-short pulsed lasers. Preliminary exploratory investigation on adequate laser parameters [J].
Bello-Silva, Marina Stella ;
Wehner, Martin ;
Eduardo, Carlos de Paula ;
Lampert, Friedrich ;
Poprawe, Reinhart ;
Hermans, Martin ;
Esteves-Oliveira, Marcella .
LASERS IN MEDICAL SCIENCE, 2013, 28 (01) :171-184
[4]   Femtosecond laser ablation properties of borosilicate glass [J].
Ben-Yakar, A ;
Byer, RL .
JOURNAL OF APPLIED PHYSICS, 2004, 96 (09) :5316-5323
[5]   The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine [J].
Bianco, Paolo ;
Cao, Xu ;
Frenette, Paul S. ;
Mao, Jeremy J. ;
Robey, Pamela G. ;
Simmons, Paul J. ;
Wang, Cun-Yu .
NATURE MEDICINE, 2013, 19 (01) :35-42
[6]   Adipose stem cells originate from perivascular cells [J].
Cai, Xiaoxiao ;
Lin, Yunfeng ;
Hauschka, Peter V. ;
Grottkau, Brian E. .
BIOLOGY OF THE CELL, 2011, 103 (09) :435-447
[7]   Femtosecond laser ablation of bovine cortical bone [J].
Cangueiro, Liliana T. ;
Vilar, Rui ;
Botelho do Rego, Ana M. ;
Muralha, Vania S. F. .
JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (12)
[8]   Adipose-derived adult stromal cells heal critical-size mouse calvarial defects [J].
Cowan, CM ;
Shi, YY ;
Aalami, OO ;
Chou, YF ;
Mari, C ;
Thomas, R ;
Quarto, N ;
Contag, CH ;
Wu, B ;
Longaker, MT .
NATURE BIOTECHNOLOGY, 2004, 22 (05) :560-567
[9]   Peripheral thermal and mechanical damage to dentin with microsecond and sub-microsecond 9.6 μm, 2.79 μm, and 0.355 μm laser pulses [J].
Dela Rosa, A ;
Sarma, AV ;
Le, CQ ;
Jones, RS ;
Fried, D .
LASERS IN SURGERY AND MEDICINE, 2004, 35 (03) :214-228
[10]   LASER-INDUCED BREAKDOWN BY IMPACT IONIZATION IN SIO2 WITH PULSE WIDTHS FROM 7 NS TO 150 FS [J].
DU, D ;
LIU, X ;
KORN, G ;
SQUIER, J ;
MOUROU, G .
APPLIED PHYSICS LETTERS, 1994, 64 (23) :3071-3073
12345