Expression of functional leptin receptors in the human ovary

被引:311
作者
Karlsson, C
Lindell, K
Svensson, E
Bergh, C
Lind, P
Billig, H
Carlsson, LMS
Carlsson, B
机构
[1] Sahlgrens Univ Hosp, Dept Internal Med, Res Ctr Endocrinol & Metab, S-41345 Gothenburg, Sweden
[2] Sahlgrens Univ Hosp, Dept Obstet & Gynecol, S-41345 Gothenburg, Sweden
[3] Gothenburg Univ, Dept Physiol & Pharmacol, Gothenburg, Sweden
[4] Pharmacia & Upjohn Inc, Dept Mol Biol, Stockholm, Sweden
关键词
D O I
10.1210/jc.82.12.4144
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The size of body fat stores is known to influence fertility, indicating a link between adipose tissue and the reproductive system. Studies in mice have identified the adipocyte-derived hormone, leptin (Ob protein), as a possible mediator of this effect. The aim of this study was to investigate the possibility that leptin may have direct effects on the human ovary. To probe this hypothesis we first analyzed the expression of leptin receptors in the human ovary. Transcripts encoding both the long and short isoforms of the leptin receptor were present in human granulosa cells and thecal cells; however, the short isoforms were expressed at much higher levels. Immunoreactive leptin was present in follicular fluid at levels similar to those found in serum. ob gene expression, however, was undetectable in the ovary, as determined by reverse transcription-PCR, whereas it was easily detected in adipose tissue. To determine whether leptin could induce a biological response in ovarian cells, we examined the effect of leptin on estradiol production in cultured granulosa cells. Leptin (100 ng/mL) inhibited LH (0.1 ng/ml)-stimulated estradiol production. In contrast, leptin had no effect on estradiol production in the absence of LH. In conclusion, this study has demonstrated that the leptin receptor is expressed in the human ovary, that leptin is present in follicular fluid, and that leptin can induce a biological response in ovarian cells. These results suggest that leptin may have a direct effect on the human ovary.
引用
收藏
页码:4144 / 4148
页数:5
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