The chondroprotective effects of ferulic acid on hydrogen peroxide-stimulated chondrocytes: inhibition of hydrogen peroxide-induced pro-inflammatory cytokines and metalloproteinase gene expression at the mRNA level

被引：61
作者：
Chen, M. P. [1 ,2 ]
Yang, S. H. [3 ]
Chou, C. H. [1 ,2 ]
Yang, K. C. [1 ,2 ]
Wu, C. C. [1 ,2 ,4 ]
Cheng, Y. H. [1 ,2 ]
Lin, Feng-Huei [1 ,2 ]
机构：
[1] Natl Taiwan Univ, Inst Biomed Engn, Coll Engn, Taipei 10051, Taiwan
[2] Natl Taiwan Univ, Inst Biomed Engn, Coll Med, Taipei 10051, Taiwan
[3] Natl Taiwan Univ Hosp, Taipei 100, Taiwan
[4] En Chu Kong Hosp, Dept Orthoped, Taipei Hsien, Taiwan
来源：
INFLAMMATION RESEARCH | 2010年 / 59卷 / 08期
关键词：
Osteoarthritis; Chondrocyte; Hydrogen peroxide; Ferulic acid; Antioxidant; LIPID-PEROXIDATION; ARTICULAR CHONDROCYTES; OXIDATIVE STRESS; TRANSCRIPTION FACTORS; RHEUMATOID-ARTHRITIS; BOVINE CHONDROCYTES; C-FOS; OSTEOARTHRITIS; CARTILAGE; OXYGEN;
D O I：
10.1007/s00011-010-0165-9
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The objective of the study is to evaluate the effect of ferulic acid (FA), an antioxidant from the Chinese herb Dong-Gui [Chinese angelica, Angelica sinensis (Oliv.) Diels], on the regulation of various genes in hydrogen peroxide-stimulated porcine chondrocytes at the mRNA level. The effect of FA and the effective concentration of FA on porcine chondrocytes was evaluated by the lactate dehydrogenase, WST-1, crystal violet assay, and a chemical luminescence assay. Gene expression in hydrogen peroxide-stimulated chondrocytes either pre- or post-treated with FA was evaluated by real-time PCR. Chondrocytes pre-treated with 40 mu M FA decreased the hydrogen peroxide-induced interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and MMP-1 and partially restored SOX9 gene expression. Post-treatment with 40 mu M FA also decreased the expression of MMP-1 and MMP-13. FA decreased the hydrogen peroxide-induced IL-1 beta, TNF-alpha, MMP-1 and MMP-13 and increased SOX9 gene expression. These findings suggest that FA may prove to be important in the treatment of osteoarthritis. Further research is needed.
引用
收藏
页码：587 / 595
页数：9
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