Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation

被引：6
作者：
Mumford, AD
Chen, D
Dorling, A
Kemball-Cook, G
McVey, JH
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Haemostasis Thrombosis MRC, Ctr Clin Sci, Fac Med, London W12 0NN, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Immunol, Fac Med, London W12 0NN, England
来源：
THROMBOSIS AND HAEMOSTASIS | 2005年 / 93卷 / 01期
基金：
英国医学研究理事会;
关键词：
tissue factor; antibody; immunisation;
D O I：
10.1160/TH03-11-0703
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Tissue factor (TF) the cellular receptor and cofactor for factor VII, initiates coagulation and has also been implicated in several coagulation-independent functions, including inflammation, angiogenesis and tumour metastasis. Investigations of TF expression in mouse models of these processes has been limited by the availability of antibodies that specifically recognise mouse TF. We have generated a rabbit polyclonal antibody to mTF by DNA immunisation. This has yielded an antiserum that recognises native mTF in immunohistochemical and flow cytometric analyses. Furthermore,the antiserum is inhibitory in coagulation assays. This antiserum will be a valuable investigative tool in the analysis of mTF expression.
引用
收藏
页码：160 / 164
页数：5
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