The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation

被引:122
作者
Antonin, W [1 ]
Franz, C [1 ]
Haselmann, U [1 ]
Antony, C [1 ]
Mattaj, IW [1 ]
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
D O I
10.1016/j.molcel.2004.12.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The metazoan nuclear envelope (NE) breaks down and reforms at each mitosis. Nuclear pore complexes (NPCs), which allow nucleocytoplasmic transport during interphase, assemble into the reforming NE at the end of mitosis. Using in vitro NE assembly assays, we show that one of the two transmembrane nucleoporins, pom121, is essential for NE formation, whereas the second, gp210, is dispensable. Depletion of either pom121-containing membrane vesicles or the protein alone does not affect vesicle binding to chromatin but prevents their fusion to form a closed NE. When the Nup107-160 complex, which is essential for integration of NPCs into the NE, is also depleted, pom121 becomes dispensable for NE formation, suggesting a close functional link between NPC and NE formation and the existence of a checkpoint that monitors NPC assembly state.
引用
收藏
页码:83 / 92
页数:10
相关论文
共 46 条
  • [1] Energy-and temperature-dependent in vitro export of RNA from synthetic nuclei
    Arts, GJ
    Englmeier, L
    Mattaj, IW
    [J]. BIOLOGICAL CHEMISTRY, 1997, 378 (07) : 641 - 649
  • [2] Bodoor K, 1999, J CELL SCI, V112, P2253
  • [3] BORDIER C, 1981, J BIOL CHEM, V256, P1604
  • [4] STEPWISE REASSEMBLY OF THE NUCLEAR-ENVELOPE AT THE END OF MITOSIS
    CHAUDHARY, N
    COURVALIN, JC
    [J]. JOURNAL OF CELL BIOLOGY, 1993, 122 (02) : 295 - 306
  • [5] Nuclear pore protein gp210 is essential for viability in HeLa cells and Caenorhabditis elegans
    Cohen, M
    Feinstein, N
    Wilson, KL
    Gruenbaum, Y
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (10) : 4230 - 4237
  • [6] COHEN M, 2001, GENE THER MOL BIOL, V6, P47
  • [7] Proteomic analysis of the mammalian nuclear pore complex
    Cronshaw, JA
    Krutchinsky, AN
    Zhang, WZ
    Chait, BT
    Matunis, MJ
    [J]. JOURNAL OF CELL BIOLOGY, 2002, 158 (05) : 915 - 927
  • [8] IDENTIFICATION OF A SOLUBLE PRECURSOR COMPLEX ESSENTIAL FOR NUCLEAR-PORE ASSEMBLY INVITRO
    DABAUVALLE, MC
    LOOS, K
    SCHEER, U
    [J]. CHROMOSOMA, 1990, 100 (01) : 56 - 66
  • [9] Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells
    Daigle, N
    Beaudouin, J
    Hartnell, L
    Imreh, G
    Hallberg, E
    Lippincott-Schwartz, J
    Ellenberg, J
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 154 (01) : 71 - 84
  • [10] IDENTIFICATION AND CHARACTERIZATION OF A NUCLEAR-PORE COMPLEX PROTEIN
    DAVIS, LI
    BLOBEL, G
    [J]. CELL, 1986, 45 (05) : 699 - 709