Clinicopathological characterization of prion: a novel marker of activated human hepatic stellate cells

被引:16
作者
Kitada, T
Seki, S
Ikeda, K
Nakatani, K
Sakaguchi, H
Kawada, N
Kadoya, H
Kaneda, K
机构
[1] Osaka City Univ, Sch Med, Dept Internal Med 3, Abeno Ku, Osaka 5458585, Japan
[2] Osaka City Univ, Sch Med, Dept Anat 2, Abeno Ku, Osaka 5458585, Japan
关键词
activated hepatic stellate cells; chronic liver disease; disease activity; prion;
D O I
10.1016/S0168-8278(00)80306-2
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: We recently demonstrated prion as a new marker for hepatic stellate cell activation in rats. Here, we have examined prion expression in normal and diseased human livers. Methods: Prion expression was examined at protein level by immunohistochemistry and at mRNA level by in situ hybridization. Results: While normal livers were negative for prion, all liver specimens but one from patients with chronic liver disease were positively stained. In chronic hepatitis, prion protein expression was found not only in the sinusoidal lining cells within the lobules but also in mesenchymal cells in expanded portal tracts. In alcoholic liver disease, prion-positive cells were found mainly in the areas of alcoholic hepatitis. Immuno-electronmicroscopy revealed that prion-positive cells were activated stellate cells. In situ hybridization demonstrated that the distribution of prion mRNA is similar to that of prion protein. In chronic hepatitis, the number of prion-positive cells correlated with the grade of activity but not with the stage of fibrosis, In alcoholic Fiver disease, levels of prion protein expression were significantly increased in the presence of alcoholic hepatitis. Conclusion: Prion as a novel marker of activated stellate cells correlates well with disease activity in human chronic liver diseases.
引用
收藏
页码:751 / 757
页数:7
相关论文
共 32 条
[1]   HUMAN MUSCLE MACROPHAGES EXPRESS BETA-AMYLOID PRECURSOR AND PRION PROTEINS AND THEIR MESSENGER-RNAS [J].
ASKANAS, V ;
SARKOZI, E ;
BILAK, M ;
ALVAREZ, RB ;
ENGEL, WK .
NEUROREPORT, 1995, 6 (07) :1045-1049
[2]  
BALLARDINI G, 1994, HEPATOLOGY, V19, P440
[3]   ROLE OF MESENCHYMAL CELL-POPULATIONS IN PORCINE SERUM INDUCED RAT-LIVER FIBROSIS [J].
BHUNCHET, E ;
WAKE, K .
HEPATOLOGY, 1992, 16 (06) :1452-1472
[4]   Spongiform encephalopathies - B lymphocytes and neuroinvasion [J].
Brown, P .
NATURE, 1997, 390 (6661) :662-663
[5]   Synaptophysin: A novel marker for human and rat hepatic stellate cells [J].
Cassiman, D ;
van Pelt, J ;
De Vos, R ;
Van Lommel, F ;
Desmet, V ;
Yap, SH ;
Roskams, T .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (06) :1831-1839
[6]  
DESMET VJ, 1994, HEPATOLOGY, V19, P1513, DOI 10.1002/hep.1840190629
[7]   Distribution and submicroscopic immunogold localization of cellular prion protein (PrPc) in extracerebral tissues [J].
Fournier, JG ;
Escaig-Haye, F ;
de Villemeur, TB ;
Robain, O ;
Lasmézas, CI ;
Deslys, JP ;
Dormont, D ;
Brown, P .
CELL AND TISSUE RESEARCH, 1998, 292 (01) :77-84
[8]  
FRIEDMAN SL, 1993, NEW ENGL J MED, V328, P1828
[9]   HEPATIC LIPOCYTES - THE PRINCIPAL COLLAGEN-PRODUCING CELLS OF NORMAL RAT-LIVER [J].
FRIEDMAN, SL ;
ROLL, FJ ;
BOYLES, J ;
BISSELL, DM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (24) :8681-8685
[10]   PERISINUSOIDAL LIPOCYTES AND FIBROGENESIS [J].
GRESSNER, AM .
GUT, 1994, 35 (10) :1331-1333