Piscidin: Antimicrobial peptide of rock bream, Oplegnathus fasciatus

被引：29

作者：

Bae, Jin-Sol ^{[1
]}

Jung, Ji-Min ^{[1
]}

An, Cheul Min ^{[2
]}

Kim, Ju-Won ^{[2
]}

Hwang, Seong Don ^{[3
]}

Kwon, Mun-Gyeong ^{[3
]}

Park, Myoung-Ae ^{[3
]}

Kim, Mu-Chan ^{[1
]}

Park, Chan-Il ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Inst Marine Ind, Coll Marine Sci, 38 Cheondaegukchi Gil, Tongyeong 650160, Gyeongnam, South Korea

[2] Natl Inst Fisheries Sci, Biotechnol Res Div, 216 Gijanghaean Ro, Busan 619705, South Korea

[3] Natl Inst Fisheries Sci, Aquat Life Dis Control Div, 216 Gijanghaean Ro, Busan 619705, South Korea

来源：

FISH & SHELLFISH IMMUNOLOGY | 2016年 / 51卷

关键词：

Rock bream; Piscidin; Antimicrobial peptides; Antimicrobial activity; Cytotoxicity; AUTOMATED PROTEIN-STRUCTURE; GENE-EXPRESSION; BIOLOGICAL-ACTIVITY; RAINBOW-TROUT; CDNA SEQUENCE; MAST-CELLS; I-TASSER; SEA BASS; FISH; PURIFICATION;

D O I：

10.1016/j.fsi.2016.02.010

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The piscidin family consists of antimicrobial peptides (AMPS) that are mainly found in fish and are crucial effectors of fish innate immune responses. The piscidin family typically has broad-spectrum antimicrobial activity and can modulate immune responses. In this study, we cloned rock bream piscidin (Rbpisc) and investigated its gene expression and biological activity (including antimicrobial and cytotoxic activities). The coding region of Rbpisc consisted of 213 base pairs (bp) encoding 70 amino acid residues. The tertiary structure predicted for Rbpisc includes an amphipathic helix-loop-helix structure. The Rbpisc gene was highly expressed in the gills of healthy fish. The gene expression of Rbpisc increased in the gills after pathogen infection, while the expression was down-regulated in other tissues. A synthetic peptide based on the AMP 12 domain amino acid sequence of Rbpisc appeared to have broad-spectrum antimicrobial activity against various bacteria. However, the synthetic peptide exhibited weak haemolytic activity against fish erythrocytes. These results suggest that Rbpisc might play an important role in the innate immune responses of rock bream. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：136 / 142

页数：7

共 63 条

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