Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields)

被引：50

作者：

Porat, Yaara ^{[1
]}

Giladi, Moshe ^{[1
]}

Schneiderman, Rosa S. ^{[1
]}

Blat, Roni ^{[1
]}

Shteingauz, Anna ^{[1
]}

Zeevi, Einav ^{[1
]}

Munster, Mijal ^{[1
]}

Voloshin, Tali ^{[1
]}

Kaynan, Noa ^{[1
]}

Tal, Orna ^{[1
]}

Kirson, Eilon D. ^{[2
]}

Weinberg, Uri ^{[3
]}

Palti, Yoram ^{[2
]}

机构：

[1] Novocure Ltd, Preclin Res Dept, Haifa, Israel

[2] Novocure Ltd, Haifa, Israel

[3] Novocure GmbH, Luzern, Switzerland

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2017年 / 123期

关键词：

Cancer Research; Issue; 123; Tumor Treating Fields; TTFields; inovitro; glioma; ovarian cancer; frequency scan; ALTERNATING ELECTRIC-FIELDS; PHYSICIANS CHOICE CHEMOTHERAPY; IN-VITRO; LUNG-CANCER; COMBINATION; DISRUPTION; VIVO; GLIOBLASTOMA; THERAPY; SYSTEM;

D O I：

10.3791/55820

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tumor Treating Fields (TTFields) are an effective treatment modality delivered via the continuous, noninvasive application of low-intensity (1-3 V/cm), alternating electric fields in the frequency range of several hundred kHz. The study of TTFields in tissue culture is carried out using the TTFields in vitro application system, which allows for the application of electric fields of varying frequencies and intensities to ceramic Petri dishes with a high dielectric constant (epsilon > 5,000). Cancerous cell lines plated on coverslips at the bottom of the ceramic Petri dishes are subjected to TTFields delivered in two orthogonal directions at various frequencies to facilitate treatment outcome tests, such as cell counts and clonogenic assays. The results presented in this report demonstrate that the optimal frequency of the TTFields with respect to both cell counts and clonogenic assays is 200 kHz for both ovarian and glioma cells.

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页数：8

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