Alternative bioseparation operations: life beyond packed-bed chromatography

被引：245

作者：

Przybycien, TM

Pujar, NS

Steele, LM

机构：

[1] Carnegie Mellon Univ, Dept Biomed Engn, Pittsburgh, PA 15213 USA

[2] Merck & Co Inc, Merck Res Labs, West Point, PA 19486 USA

[3] Genencor Int, Palo Alto, CA 94304 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2004年 / 15卷 / 05期

关键词：

D O I：

10.1016/j.copbio.2004.08.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attractive proposition, even if only a reduction in the extent of use of packed beds can be realized. This paper reviews the current state of unit operations posing as chromatography alternatives - including membrane filtration, aqueous two-phase extraction, three-phase partitioning precipitation, crystallization, monoliths and membrane chromatography - and their potential to do the unthinkable.

引用

页码：469 / 478

页数：10

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