Expression of lactate dehydrogenase is induced during hypoxia via HIF-1 in the mud crab Scylla paramamosain

被引：27

作者：

Xie, Jiawei ^{[1
,2
]}

Cheng, Changhong ^{[1
]}

Jie, Yukun ^{[1
,2
]}

Ma, Hongling ^{[1
]}

Feng, Juan ^{[1
]}

Su, Youlu ^{[1
]}

Deng, Yiqin ^{[1
]}

Xu, Haidong ^{[1
]}

Guo, Zhixun ^{[1
,2
]}

机构：

[1] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc Key Lab South China Sea F, Minist Agr, Guangzhou 510300, Guangdong, Peoples R China

[2] Shanghai Ocean Univ, Shanghai 201206, Peoples R China

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2019年 / 225卷

关键词：

Scylla paramamosain; Hypoxia-inducible factor-1 alpha; Lactate dehydrogenase; Hypoxia; Gene silencing; ENERGY-METABOLISM; GENE-EXPRESSION; SHRIMP; MECHANISM; OXYGEN; EVOLUTION; PEPTIDES; PROTEINS; ANOXIA; MUSCLE;

D O I：

10.1016/j.cbpc.2019.108563

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lactate dehydrogenase (LDH) is a key enzyme involved in anaerobic metabolism in most organisms. In the present study, we determined the structure and function of LDH sequence in Scylla paramamosain (SpLDH) by gene cloning, expression and RNA interference techniques in order to explore the genetic characteristics of LDH and its relationship with HIF-1 during hypoxia. The full-length cDNA was 1453 bp with an open reading frame (ORF) of 996 bp, and encoded a polypeptide of 332 amino acids. Homology analysis showed that the SpLDH gene is highly similar to arthropods. The SpLDH transcript increased after hypoxia in all tested tissues. The silencing of HIF-1 blocked the increase in LDH mRNA and activity, which were induced by hypoxia in gill and muscle tissues. Our results indicated that SpLDH expression was regulated transcriptionally by HIF-1.

引用

页数：9

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