Increased Expression of Matrix Metalloproteinase-9 in Patients with Temporal Lobe Epilepsy

被引:27
作者
Acar, Goksemin [1 ]
Tanriover, Gamze [2 ]
Acar, Feridun [3 ]
Demir, Ramazan [2 ]
机构
[1] Pamukkale Univ, Sch Med, Dept Neurol, Denizli, Turkey
[2] Akdeniz Univ, Sch Med, Dept Histol & Embryol, TR-07058 Antalya, Turkey
[3] Pamukkale Univ, Sch Med, Dept Neurosurg, Denizli, Turkey
关键词
Hippocampal sclerosis; Matrix metalloproteinase-9; Temporal lobe epilepsy; LONG-TERM POTENTIATION; MATRIX METALLOPROTEINASES; SPONTANEOUS SEIZURES; STATUS EPILEPTICUS; RAT-BRAIN; EPILEPTOGENESIS; PLASTICITY; HIPPOCAMPUS; ACTIVATION; CORTEX;
D O I
10.5137/1019-5149.JTN.10738-14.0
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
AIM:The molecular mechanism of epileptogenesis in temporal lobe epilepsy is still unclear. Experimental studies have suggested that matrix metalloproteinases have important roles in this process, but human studies are limited. The aim of this study was to assess the expression of MMP-9, MMP-2 and their tissue inhibitors (TIMP-1 and TIMP-2) in patients with temporal lobe epilepsy with hippocampal sclerosis (TLE-HS). MATERIAL and METHODS: The tissue samples from temporal neocortex and hippocampus were obtained from patients with temporal lobe epilepsy with hippocampal sclerosis who had undergone anterior temporal lobectomy for recurrent medically resistant seizures. Immunohistochemical methods were used to determine the expression of MMP-9, MMP-2 and their tissue inhibitors. Tissue samples were also analyzed with transmission electron microscopy. RESULTS: The immunoreactivity for MMP-9 both in hippocampal and temporal neocortical neurons was stronger than that of MMP-2. Additionally, there was a mild reaction for its tissue inhibitor TIMP-1 as with TIMP-2. The TEM analysis of the hippocampus revealed that there was apparent ultra-structural damage on the pericarya and neuropil of some neurons. There was obvious damage in the mitochondria and the nuclear membrane. CONCLUSION:The preliminary results of this study revealed that MMP-9 may have a role in patients with drug resistant TLE-HS.
引用
收藏
页码:749 / 756
页数:8
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