Robust IR-based detection of stable and fractionally populated G-C+ and A-T Hoogsteen base pairs in duplex DNA

被引：12

作者：

Stelling, Allison L. ^{[1
]}

Xu, Yu ^{[2
]}

Zhou, Huiqing ^{[1
]}

Choi, Seung H. ^{[1
]}

Clay, Mary C. ^{[1
]}

Merriman, Dawn K. ^{[2
]}

Al-Hashimi, Hashim M. ^{[1
,2
]}

机构：

[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

[2] Duke Univ, Dept Chem, Durham, NC 27706 USA

来源：

FEBS LETTERS | 2017年 / 591卷 / 12期

关键词：

DNA; Hoogsteen; infrared; m1A; m1G; NMR; ECHINOMYCIN BINDING-SITES; NORMAL COORDINATE ANALYSIS; HYDROGEN-BONDED COMPLEX; RESONANCE RAMAN BANDS; WATSON-CRICK; NUCLEIC-ACIDS; VIBRATION MODES; CONFORMATIONAL-CHANGES; MOLECULAR-STRUCTURE; TELOMERIC DNA;

D O I：

10.1002/1873-3468.12681

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Noncanonical G-C+ and A-T Hoogsteen base pairs can form in duplex DNA and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in DNA duplexes remains challenging due to difficulties in resolving syn versus antipurine bases with X-ray crystallography; and size limitations and line broadening can make them difficult to characterize by NMR spectroscopy. Here, we show how infrared (IR) spectroscopy can identify G-C+ and A-T Hoogsteen base pairs in duplex DNA across a range of different structural contexts. The utility of IR-based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A-T and G-C+ Hoogsteen base pairs in a DNA duplex where severe broadening complicates detection with NMR.

引用

页码：1770 / 1784

页数：15

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