Transesterification catalyzed by trypsin supported on MCM-41

被引：30

作者：

Gómez, JM

Deere, J

Goradia, D

Cooney, J

Magner, E

Hodnett, BK ^{[1
]}

机构：

[1] Univ Limerick, Mat & Surface Sci Inst, Limerick, Ireland

[2] Univ Limerick, Dept Chem & Environm Sci, Limerick, Ireland

来源：

CATALYSIS LETTERS | 2003年 / 88卷 / 3-4期

关键词：

transesterification; trypsin; enzyme; MCM-41; mesoporous; stability;

D O I：

10.1023/A:1024057605239

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Trypsin supported on MCM-41 is demonstrated as a stable catalyst for the transesterfication of N-acetyl-L-tyrosine ethyl ester with propan-1-ol. An enzyme loading of 5 micromoles of trypsin per gram of silicate was readily achieved. The ultimate enzyme loading was shown to depend strongly on enzyme purity. The adsorbed enzyme exhibited the same turnover frequency for the transesterification reaction as native trypsin, the catalyst could be reused without loss of activity following separation by centrifuging and the enzyme did not leach during testing.

引用

收藏

页码：183 / 186

页数：4

相关论文

共 14 条

[1] A NEW FAMILY OF MESOPOROUS MOLECULAR-SIEVES PREPARED WITH LIQUID-CRYSTAL TEMPLATES [J].

BECK, JS ;

VARTULI, JC ;

ROTH, WJ ;

LEONOWICZ, ME ;

KRESGE, CT ;

SCHMITT, KD ;

CHU, CTW ;

OLSON, DH ;

SHEPPARD, EW ;

MCCULLEN, SB ;

HIGGINS, JB ;

SCHLENKER, JL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (27) :10834-10843

[2] Mechanistic and structural features of protein adsorption onto mesoporous silicates [J].

Deere, J ;

Magner, E ;

Wall, JG ;

Hodnett, BK .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (29) :7340-7347

[3] Enzyme immobilization in MCM-41 molecular sieve [J].

Diaz, JF ;

Balkus, KJ .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) :115-126

[4]

Gimon-Kinsel ME, 1998, STUD SURF SCI CATAL, V117, P373

[5] Catalytic activity of mesoporous silicate-immobilized chloroperoxidase [J].

Han, YJ ;

Watson, JT ;

Stucky, GD ;

Butler, A .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 17 (01) :1-8

[6]

He J, 2000, CHINESE CHEM LETT, V11, P45

[7] ORDERED MESOPOROUS MOLECULAR-SIEVES SYNTHESIZED BY A LIQUID-CRYSTAL TEMPLATE MECHANISM [J].

KRESGE, CT ;

LEONOWICZ, ME ;

ROTH, WJ ;

VARTULI, JC ;

BECK, JS .

NATURE, 1992, 359 (6397) :710-712

[8] A method for the synthesis of high quality large crystal MCM-41 [J].

Mokaya, R ;

Zhou, WZ ;

Jones, W .

CHEMICAL COMMUNICATIONS, 1999, (01) :51-52

[9] Catalytic activity in organic solvents and stability of immobilized enzymes depend on the pore size and surface characteristics of mesoporous silica [J].

Takahashi, H ;

Li, B ;

Sasaki, T ;

Miyazaki, C ;

Kajino, T ;

Inagaki, S .

CHEMISTRY OF MATERIALS, 2000, 12 (11) :3301-3305

[10] Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent [J].

Takahashi, H ;

Li, B ;

Sasaki, T ;

Miyazaki, C ;

Kajino, T ;

Inagaki, S .

MICROPOROUS AND MESOPOROUS MATERIALS, 2001, 44 :755-762