An improved method for the detection of Tospoviruses using the polymerase chain reaction

被引：54
作者：
Mumford, RA
Barker, I
Wood, KR
机构：
[1] UNIV BIRMINGHAM,SCH BIOL SCI,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
[2] CENT SCI LAB,HARPENDEN AL5 2BD,HERTS,ENGLAND
来源：
JOURNAL OF VIROLOGICAL METHODS | 1996年 / 57卷 / 01期
关键词：
tomato spotted wilt virus; impatiens necrotic spot virus; tomato chlorotic spot virus; groundnut ringspot virus; RT-PCR; Tospoviruses; plant viral diagnostics;
D O I：
10.1016/0166-0934(95)01975-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.
引用
收藏
页码：109 / 115
页数：7
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