Effect of Datura stramonium on Cyclophosphamide-induced Oxidative Stress in Albino Rats: Study on Kidney Markers

Background: Cyclophosphamide is a chemotherapeutic agent, whose activation induces oxidative stress, causing nephrotoxicity. The present study ascertained the effect of the aqueous extracts of Datura stramonium seeds and leaves on cyclophosphamide-induced oxidative stress in rats. Materials and Methods: Twenty-four Wistar albino rats were divided into 6 groups of 4 rats each. All groups, except group 1, were induced with cyclophosphamide at a dose of 150 mg kg(-1) b.wt. Group 1 was the normal control, group 2 (positive control) was left untreated, group 3 and 4 were administered 200 and 400 mg kg(-1) b.wt., of the seed extract respectively, while group 5 and 6 were administered 200 and 400 mg kg(-1) b.wt., of the leaf extract respectively, after cyclophosphamide induction. Results: Administration of the extracts caused significant (p<0.05) decreases in catalase and glutathione peroxidase, glutathione, malondialdehyde and urea concentrations compared to group 2.The extract also led to significant (p<0.05) increases in albumin, globulin, total protein, vitamins C and E concentrations compared to group 2. However, there was no significant difference in vitamin A concentration after extract ad ministration. Conclusion:This study showed the ability of Datura stramonium in restoring oxidative stress induced by cyclophosphamide, though not all indices considered were significantly reversed.