Guidelines for reporting of biocatalytic reactions

被引:118
作者
Gardossi, Lucia [2 ]
Poulsen, Pout B. [3 ]
Ballesteros, Antonio [4 ]
Hult, Karl [5 ]
Svedas, Vytas K. [6 ]
Vasic-Racki, Durda [7 ]
Carrea, Giacomo [8 ]
Magnusson, Anders [9 ]
Schmid, Andreas [9 ]
Wohlgemuth, Roland [10 ]
Halling, Peter J. [1 ]
机构
[1] Univ Strathclyde, Dept Pure & Appl Chem, WestCHEM, Glasgow G1 1XL, Lanark, Scotland
[2] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy
[3] Novozymes AS, DK-2880 Bagsvaerd, Denmark
[4] CSIC, Dept Biocatalysis, Inst Catalysis, Madrid, Spain
[5] AlbaNova Univ Ctr, Sch Biotechnol, Royal Inst Technol KTH, Dept Biochem, SE-10691 Stockholm, Sweden
[6] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Fac Bioengn & Bioinformat, Moscow 119991, Russia
[7] Univ Zagreb, Fac Chem Engn & Technol, Zagreb 10000, Croatia
[8] CNR, Ist Chim Riconoscimento Mol, Lab Chem Biotechnol, I-20131 Milan, Italy
[9] TU Dortmund Univ, Dept Biochem & Chem Engn, D-44227 Dortmund, Germany
[10] Sigma Aldrich, Res Specialt, CH-9470 Buchs, Switzerland
基金
英国工程与自然科学研究理事会;
关键词
PENICILLIN ACYLASE; ENZYME;
D O I
10.1016/j.tibtech.2010.01.001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enzymes and whole cells are being increasingly applied in research and industry, but the adoption of biocatalysis relies strongly on useful scientific literature. Unfortunately, too many published papers lack essential information needed to reproduce and understand the results. Here, members of the scientific committee of the European Federation of Biotechnology Section on Applied Biocatalysis (ESAB) provide practical guidelines for reporting experiments. The document embraces the recommendations of the STRENDA initiative (Standards for Reporting Enzymology Data) in the context of pure enzymology and provides further guidelines and explanations on topics of crucial relevance for biocatalysis. In particular, guidelines are given on issues such as the selectivity, specificity, productivity and stability of biocatalysts, as well as on methodological problems related to reactions in multiphase systems. We believe that adoption and use of these guidelines could greatly increase the value and impact of published work in biocatalysis, and hence promote the further growth of applications.
引用
收藏
页码:171 / 180
页数:10
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