Luteolibacter marinus sp. nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment

A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sedi-ment sampled on Meishan Island located in the East China Sea. Strain NBU1238(T) was able to grow optimally at 28-32 degrees C, at pH 7.5 and with no NaCl. Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive. Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative. The major cellular fatty acids were iso-C-14:0, C-16:0, C-16:1 ?9c and C-14:0. The only respiratory quinone was menaquinone- 9. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The 16S rRNA gene sequence of strain NBU1238(T) showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKX(T), Luteolibacter luteus G-1-1-1(T), Luteolibacter arcticus MC 3726(T) and Luteolibacter pohnpeiensis A4T-83(T), respectively. Phylogenetic analyses indicated that strain NBU1238(T) clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKX(T), L. arcticus MC 3726(T) and L. luteus G-1-1-1(T). The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238(T) and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of the genomic DNA was 65.0 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238(T) is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp. nov. is proposed. The type strain is NBU1238(T) (=KCTC 82227(T)=MCCC 1K04772(T)).