Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor

被引：11

作者：

Billing, T. M. ^{[1
]}

Guay, P.-J.

Peucker, A. J.

Mulder, R. A.

机构：

[1] Univ Melbourne, Dept Zool, Melbourne, Vic 3010, Australia

[2] Deakin Univ, Sch Life & Environm Sci, Warrnambool, Vic 3280, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 03期

关键词：

Eudyptula minor; little penguin; microsatellite; parentage; paternity; population genetics;

D O I：

10.1111/j.1471-8286.2006.01607.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated and characterized eight novel microsatellite loci in the little penguin Eudyptula minor, using nonradioactive polymerase chain reaction-based techniques to screen GA and GAAA repeats from enriched genomic DNA libraries. All eight loci were polymorphic and seven were variable in our main study population (mean H-E = 0.613, mean N-A = 7.14). Cross-amplification using a microsatellite primer developed in Spheniscus demersus (African penguin) yielded one additional polymorphic locus. This locus combined with six of the little penguin loci is suitable for paternity assignment in little penguins (exclusion probability for seven unlinked loci = 0.993).

引用

页码：425 / 427

页数：3

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