Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

被引：11

作者：

Dwyer, Connor J. ^{[1
]}

Cloud, Colleen A. ^{[1
]}

Wang, Cindy ^{[1
]}

Heidt, Philip ^{[1
]}

Chakraborty, Paramita ^{[1
]}

Duke, Tara F. ^{[1
]}

McGue, Shannon ^{[2
]}

Jeffcoat, Braxton ^{[1
]}

Dunne, Jaclyn ^{[1
]}

Johnson, Logan ^{[1
]}

Choi, Seungho ^{[1
]}

Nahhas, Georges J. ^{[3
]}

Gandy, Amy S. ^{[4
]}

Babic, Nikolina ^{[5
]}

Nolte, Frederick S. ^{[5
]}

Howe, Philip ^{[6
]}

Ogretmen, Besim ^{[6
]}

Gangaraju, Vamsi K. ^{[6
]}

Tomlinson, Stephen ^{[7
]}

Madden, Brian ^{[8
]}

Bridges, Tracy ^{[8
]}

Flume, Patrick A. ^{[2
]}

Wrangle, John ^{[2
]}

Rubinstein, Mark P. ^{[1
]}

Baliga, Prabhakar K. ^{[1
]}

Nadig, Satish N. ^{[1
,10
]}

Mehrotra, Shikhar ^{[1
,9
]}

机构：

[1] Med Univ South Carolina, Dept Surg, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[2] Med Univ South Carolina, Dept Med, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[3] Med Univ South Carolina, Dept Psychiat & Behav Sci, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[4] Med Univ South Carolina, Clin & Translat Res Ctr, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[5] Med Univ South Carolina, Lab & Pathol Med, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[6] Med Univ South Carolina, Biochem & Mol Biol, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[7] Med Univ South Carolina, Microbiol & Immunol, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[8] Blood Connect, 1099 Bracken Rd, Piedmont, SC 29673 USA

[9] Med Univ South Carolina, Dept Surg Dermatol Microbiol & Immunol, Hollings Canc Ctr HO 512H, 86 Jonathan Lucas St, Charleston, SC 29425 USA

[10] Dept Surg Pediat Microbiol & Immunol, 96 Jonathan Lucas St,MSC 613 CSB 409, Charleston, SC 29425 USA

来源：

ISCIENCE | 2021年 / 24卷 / 06期

关键词：

SEVERITY; IMMUNITY;

D O I：

10.1016/j.isci.2021.102489

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3% were positive. Of these 3%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.

引用

页数：13

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