Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans

被引：72

作者：

Ben Arous, Juliette ^{[1
]}

Tanizawa, Yoshinori ^{[2
]}

Rabinowitch, Ithai ^{[2
]}

Chatenay, Didier ^{[3
]}

Schafer, William R. ^{[2
]}

机构：

[1] UPMC, CNRS, Ecole Normale Super, Lab Phys Stat, F-75005 Paris, France

[2] MRC, Mol Biol Lab, Div Cell Biol, Cambridge CB2 0QH, England

[3] UPMC, CNRS, FRE 3231, Lab Jean Perrin, F-75005 Paris, France

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2010年 / 187卷 / 02期

基金：

以色列科学基金会;

关键词：

Behavior; Calcium imaging; C; elegans; Tracking system; Mechanosensory neurons; C-ELEGANS; CA2+ INDICATORS; CIRCUIT; INTEGRATION; CALMODULIN; LOCOMOTION; WORM;

D O I：

10.1016/j.jneumeth.2010.01.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to understand how neuronal circuits control locomotory patterns it is necessary to record neuronal activity of freely behaving animals. Here, using a new automated system for simultaneous recording of behavior and neuronal activity in freely moving Caenorhabditis elegans on standard agar plates, we show that spontaneous reversals from forward to backward locomotion reflect precisely the activity of the AVA command interneurons. We also witness spontaneous activity transients in the PLM sensory neurons during free behavior of the worm in standard conditions. We show that these activity transients are coupled to short spontaneous forward accelerations of the worm. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：229 / 234

页数：6

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