Relationship between plasma membrane redox system and elongation of hypocotyls in mungbean (Phaseolus radiatus L.) under drought stress

被引:0
作者
Gong, YH [1 ]
Gao, JF [1 ]
Shangguan, ZP [1 ]
Liang, ZS [1 ]
Shao, MG [1 ]
机构
[1] NW Sci Tech Univ Agr & Forestry, Coll Life Sci, Shaanxi 712100, Peoples R China
来源
WATER-SAVING AGRICULTURE AND SUSTAINABLE USE OF WATER AND LAND RESOURCES, VOLS 1 AND 2, PROCEEDINGS | 2004年
关键词
drought stress; elongation growth; plasma membrane redox system; H+ extrusion; mungbean (Phaseolus radiatus L.);
D O I
暂无
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
An osmotic agent, -0.9 MPa D-Mannitol, imitating drought stress in mungbean roots, was used to investigate the relationship between redox system in plant. plasma membrane and elongation growth under drought stress. Results showed that -0.9 Mpa D-Mannitol significantly similarly inhibited the elongation, H+ extrusion and NADH oxidative activity in the growing zone of mungbean hypocotyl. Within 24 hours, they were reduced by D-Mannitol up to 49.1%, 44.1% and 49.2%, respectively. 1.0cm long hypocotyl segments from the seedlings grown in DI water (control) were floated for 12h in the dark at room temperature in 4 solutions respectively: CK0 (pH7.0 buffer only), N(+ 0.2 mmol(.)L(-1)NADH), D(+0.02 mmol(.)L(-1) Actinomycin D) and ND(NADH + Actinomycin D); The hypocotyl segments from the seedlings grown in -0.9 MPa D-Mannitol (treatment for 24h) were floated for 12h in other 4 solutions correspondingly, and all 4 contained -0.9 MPa a Mannitol in pH7.0 buffer. Then the length of hypocotyls and pH of solution were assayed. Actinomycin D, the specific inhibitor of NADH oxidase, could inhibit growth of isolated hypocotyl by 41.2% (control) and 31.8% (treatment) respectively. Applied NADH could enhance their growth up to 27.8% (control) and 14.2% (treatment) respectively, but such effect could be counteracted by actinomycin D. Under drought stress, H+ extrusion rate were reduced 34.4% by Actinomycin D. Applied NADH could enhance H+ extrusion to 20% (control) and 14% (treatment), respectively. Under drought stress, inhibited elongation growth of mungbean hypocotyls may relate to the NADH oxidase in the PM redox system, with the regulation role being carried out through H+ extrusion.
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页码:169 / 175
页数:7
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