Molecular dimensions of Hb-based O2 carriers determine constriction of resistance arteries and hypertension

被引:165
作者
Sakai, H
Hara, H
Yuasa, M
Tsai, AG
Takeoka, S
Tsuchida, E [1 ]
Intaglietta, M
机构
[1] Waseda Univ, Adv Res Inst Sci & Engn, Dept Polymer Chem, Tokyo 1698555, Japan
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2000年 / 279卷 / 03期
关键词
blood substitutes; resistance vessels; microcirculation; autoregulation; nitric oxide; hemoglobin;
D O I
10.1152/ajpheart.2000.279.3.H908
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The effect of molecular dimension of hemoglobin (Hb)-based O-2 carriers on the diameter of resistance arteries (A(0), 158 +/- 21 mu m) and arterial blood pressure were studied in the conscious hamster dorsal skinfold model. Cross-linked Hb (XLHb), polyethylene glycol (PEG)-conjugated Hb, hydroxyethylstarch-conjugated XLHb, polymerized XLHb, and PEG-modified Hb vesicles (PEG-HbV) were synthesized. Their molecular diameters were 7, 22, 47, 68, and 224 nm, respectively. The bolus infusion of 7 ml/kg of XLHb (5 g/dl) caused an immediate hypertension (+34 +/- 13 mmHg at 3 h) with a simultaneous decrease in A(0) diameter (79 +/- 8% of basal value) and a blood flow decrease throughout the microvascular network. The diameter of smaller arterioles did not change significantly. Infusion of larger O-2 carriers resulted in lesser vasoconstriction and hypertension, with PEG-HbV showing the smallest changes. Constriction of resistance arteries was found to be correlated with the level of hypertension, and the responses were proportional to the molecular dimensions of the O-2 carriers. The underlying mechanism is not evident from these experiments; however, it is likely that the effects are related to the diffusion properties of the different Hb molecules.
引用
收藏
页码:H908 / H915
页数:8
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