HIV-1 exposed uninfected men who have sex with men have increased levels of salivary CC-chemokines associated with sexual behavior

被引：9

作者：

Hasselrot, Klara ^{[1
]}

Bratt, Goran ^{[2
]}

Duvefelt, Kristina ^{[3
]}

Hirbod, Taha ^{[1
]}

Sandstrom, Eric ^{[2
]}

Broliden, Kristina ^{[1
]}

机构：

[1] Karolinska Inst, Karolinska Univ Hosp, Ctr Mol Med, Dept Med,Solna Infect Dis Unit, S-17176 Stockholm, Sweden

[2] Karolinska Inst, Dept Clin Sci & Educ, Sodersjukhuset, Unit Venhalsan, S-17176 Stockholm, Sweden

[3] Karolinska Inst, MAF Novum, Huddinge, Sweden

来源：

AIDS | 2010年 / 24卷 / 10期

基金：

瑞典研究理事会;

关键词：

CC-chemokines; defensins; exposed uninfected individuals; genotyping; IgA2; LL-37; men who have sex with men; secretory leukocyte peptidase inhibitor; single nucleotide polymorphism; IMMUNODEFICIENCY-VIRUS TYPE-1; TRANSMISSION; INFECTION; INDIVIDUALS; RESISTANCE; ALLOIMMUNIZATION; MUCOSAL; PLASMA; GENE; IGA;

D O I：

10.1097/QAD.0b013e32833ac646

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objectives: To determine whether soluble molecules with known anti-HIV-1 activity are increased in saliva of HIV-1 exposed uninfected individuals of discordant couples of men who have sex with men (MSM), and whether the levels of these molecules are associated with genetic polymorphisms, sexual behavior and/or HIV-1 neutralizing capacity. Methods: Saliva and PBMC were collected from exposed uninfected individuals (n = 25), and low-risk controls (n = 22). Levels of CCL2, CCL3, CCL4, CCL5 and CCL11 were detected by Luminex, and SLPI, LL-37, alpha-defensins and IgA2 were detected by ELISA. Single nucleotide polymorphisms (SNPs) were investigated using mass spectrometry or PCR-sequencing. HIV-1 neutralizing activity was assessed using PBMC-based neutralization assays. Self-reported questionnaires described sexual behavior. Results: Exposed uninfected individuals had significantly higher levels of salivary CCL2, CCL4, CCL5 and CCL11 as compared with controls although genetic polymorphisms within the corresponding regions were equally distributed. IgA2 was also increased in exposed uninfected individuals, whereas neither CCL3, SLPI, LL-37 nor alpha-defensins differed between exposed uninfected individuals and controls. The HIV-1 neutralizing capacity of saliva was associated with higher levels of CC-chemokines (but not SLPI, LL-37, alpha-defensins or IgA2) in both exposed uninfected individuals and controls. The increased levels of CC-chemokines were associated with a higher frequency of unprotected oral sex and/or additional casual sex partners. Conclusion: HIV-1 exposed uninfected MSM had higher levels of salivary CC-chemokines compared with controls, this finding associated with sexual behavior rather than with genetic polymorphisms. The increased levels of CC-chemokines associated with HIV-1 neutralizing capacity in saliva. (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins

引用

页码：1569 / 1575

页数：7

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