Enhancement of Wound Healing by Non-Thermal N2/Ar Micro-Plasma Exposure in Mice with Fractional-CO2-Laser-Induced Wounds

被引：32

作者：

Shao, Pei-Lin ^{[1
,7
]}

Liao, Jiunn-Der ^{[1
,2
]}

Wong, Tak-Wah ^{[3
]}

Wang, Yi-Cheng ^{[1
]}

Leu, Steve ^{[4
,5
]}

Yip, Hon-Kan ^{[4
,5
,6
]}

机构：

[1] Natl Cheng Kung Univ, Dept Mat Sci & Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Med Device Innovat Ctr, Tainan 70101, Taiwan

[3] Natl Cheng Kung Univ, Dept Dermatol, Dept Biochem & Mol Biol, Med Coll & Hosp, Tainan 70101, Taiwan

[4] Kaohsiung Chang Gung Mem Hosp, Ctr Translat Res Biomed Sci, Kaohsiung 83301, Taiwan

[5] Chang Gung Univ, Coll Med, Kaohsiung 83301, Taiwan

[6] Kaohsiung Chang Gung Mem Hosp, Div Cardiol, Dept Internal Med, Kaohsiung 83301, Taiwan

[7] Asia Univ, Dept Nursing, Taichung 41354, Taiwan

来源：

PLOS ONE | 2016年 / 11卷 / 06期

关键词：

OPTICAL COHERENCE TOMOGRAPHY; NITRIC-OXIDE; HUMAN SKIN; REPAIR; STERILIZATION; ANGIOGENESIS; REGENERATION; MECHANISMS; EXPRESSION; MIGRATION;

D O I：

10.1371/journal.pone.0156699

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically.

引用

页数：15