Jungle on the Electrode: A Target-Induced Enzyme-Free and Label-Free Biosensor

被引：10

作者：

Chen, Xian ^{[1
]}

Liu, Yaoze ^{[1
]}

Xu, Liming ^{[1
]}

Wang, Yang ^{[1
]}

Li, Rui ^{[1
]}

Sun, Pengming ^{[2
]}

Lin, Zhenyu ^{[1
]}

Yang, Huanghao ^{[1
]}

机构：

[1] Fuzhou Univ, Coll Chem, Fuzhou 350116, Fujian, Peoples R China

[2] Fujian Med Univ, Affiliated Hosp, Fujian Prov Matern & Childrens Hosp, Fuzhou 350001, Fujian, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 21期

基金：

中国国家自然科学基金;

关键词：

ELECTROCHEMILUMINESCENCE BIOSENSOR; AMPLIFICATION STRATEGY; SENSITIVE DETECTION; DNA; POLYMERASE; MICRORNAS; NANOMACHINE; RECOGNITION; MECHANISM; CASCADE;

D O I：

10.1021/acs.analchem.9b03004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

With the development of clinical medicine related technologies, the detection of cancer biomarkers has become an essential method for cancer diagnosis. DNA self-assembly is a valuable approach to monitor low-abundance miRNA. Herein, we report a novel DNA jungle biosensor for target-induced enzyme-free and label-free ultrasensitive detection of miRNA-21 in biological samples. The method consists of two parts. The target catalyzes the assembly of the hairpin to form the backbone of the DNA jungle, and the auxiliary probes hybridize to form branches freely and undirectedly. The DNA jungle can be self-assembled seeded from a single target initiator, affording the potential for screening a single target molecule. Vitro assays show that the DNA jungle offers very high amplification efficiency and specificity, with a direct detection limit of 1 aM. This DNA jungle strategy can provide a useful platform for low-abundance biomarker detection for cell biology and diagnostics.

引用

页码：13712 / 13719

页数：8

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