Amperometric immunosensor for ricin by using on graphite and carbon nanotube paste electrodes

被引：33

作者：

Suresh, S. ^{[1
]}

Gupta, A. K. ^{[1
]}

Rao, V. K. ^{[1
]}

Kumar, Om ^{[1
]}

Vijayaraghavan, R. ^{[1
]}

机构：

[1] Def Res & Dev Estab, BD Div, Gwalior 474002, Madhya Pradesh, India

来源：

TALANTA | 2010年 / 81卷 / 1-2期

关键词：

Ricin; Multiwalled carbon nanotube; Carbon paste electrodes; Amperometry; Immunosensor; ELISA; ELECTROCHEMICAL IMMUNOASSAY; SALMONELLA-TYPHI; PURIFICATION; ROUTE; ASSAY;

D O I：

10.1016/j.talanta.2010.01.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ricin is a scheduled chemical warfare agent and biological warfare agent. Attempts were made for the detection of ricin in water samples by utilizing amperometric immunosensors. These electrodes were made by mixing Paraffin oil with graphite powder and multiwalled carbon nanotubes. The graphite paste electrode (CPE) and multiwalled carbon nanotubes paste electrode (MWCNTPE) were tested for their ability to detect 1-naphthol. A sandwich enzyme linked immunosorbent assay system was used to detect ricin. The detection limit for both electrodes was compared. It was found that the response of amperometric sensor is proportional to the ricin concentration in both the cases and is linear in the range 0.625-25 ng/ml for MWCNTPE and 2.5-25 ng/ml for CPE. The SEM showed that the MWCNTPE has revealed crevices/voids in which the antibodies may get trapped. Spectroscopic experiments proved that MWCNTPE adsorbs antibodies better than CPE. The high sensitivity of MWCNTPE was attributed to its better electrochemical properties rather than to its efficiency to adsorb antibodies. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：703 / 708

页数：6

共 43 条

[11] IDENTIFICATION AND QUANTIFICATION OF RICIN TOXIN IN ANIMAL-TISSUES USING ELISA [J].

GRIFFITHS, GD ;

NEWMAN, H ;

GEE, DJ .

JOURNAL OF THE FORENSIC SCIENCE SOCIETY, 1986, 26 (05) :349-358

[12] INHALATION TOXICOLOGY AND HISTOPATHOLOGY OF RICIN AND ABRIN TOXINS [J].

GRIFFITHS, GD ;

RICE, P ;

ALLENBY, AC ;

BAILEY, SC ;

UPSHALL, DG .

INHALATION TOXICOLOGY, 1995, 7 (02) :269-288

[13] OPENING AND PURIFICATION OF CARBON NANOTUBES IN HIGH YIELDS [J].

HIURA, H ;

EBBESEN, TW ;

TANIGAKI, K .

ADVANCED MATERIALS, 1995, 7 (03) :275-276

[14] Molecular recognition of biowarfare agents using micromechanical sensors [J].

Ji, HF ;

Yan, XD ;

Zhang, J ;

Thundat, T .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2004, 4 (06) :859-866

[15] Carbon nanotube/gold nanoparticles/polyethylenimine-functionalized ionic liquid thin film composites for glucose biosensing [J].

Jia, Fei ;

Shan, Changsheng ;

Li, Fenghua ;

Niu, Li .

BIOSENSORS & BIOELECTRONICS, 2008, 24 (04) :945-950

[16]

KARTHIK S, 2005, SENSOR ACTUAT B-CHEM, V107, P640

[17] ENZYME-LINKED IMMUNOASSAY OF RICIN [J].

KOJA, N ;

SHIBATA, T ;

MOCHIDA, K .

TOXICON, 1980, 18 (5-6) :611-618

[18] Oxidative stress associated hepatic and renal toxicity induced by ricin in mice [J].

Kumar, O ;

Sugendran, K ;

Vijayaraghavan, R .

TOXICON, 2003, 41 (03) :333-338

[19] Comparison of the electrochemical reactivity of electrodes modified with carbon nanotubes from different sources [J].

Lawrence, NS ;

Deo, RP ;

Wang, J .

ELECTROANALYSIS, 2005, 17 (01) :65-72

[20]

LI M, 2008, BIOPROC BIOSYST ENG, V31, P55

← 1 2 3 4 5 →