Generation of Antibodies against Foot-and-Mouth-Disease Virus Capsid Protein VP4 Using Hepatitis B Core VLPs as a Scaffold

被引:9
作者
Swanson, Jessica [1 ,2 ]
Fragkoudis, Rennos [1 ,3 ]
Hawes, Philippa C. [1 ]
Newman, Joseph [1 ]
Burman, Alison [1 ]
Panjwani, Anusha [1 ]
Stonehouse, Nicola J. [2 ]
Tuthill, Tobias J. [1 ]
机构
[1] Pirbright Inst, Pirbright GU24 0NF, England
[2] Univ Leeds, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England
[3] Univ Nottingham, Sch Vet Med & Sci, Loughborough NG7 2RD, Leics, England
来源
LIFE-BASEL | 2021年 / 11卷 / 04期
基金
英国生物技术与生命科学研究理事会;
关键词
picornavirus; FMDV; VLP; capsid; antibodies;
D O I
10.3390/life11040338
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The picornavirus foot-and-mouth disease virus (FMDV) is the causative agent of the economically important disease of livestock, foot-and-mouth disease (FMD). VP4 is a highly conserved capsid protein, which is important during virus entry. Previous published work has shown that antibodies targeting the N-terminus of VP4 of the picornavirus human rhinovirus are broadly neutralising. In addition, previous studies showed that immunisation with the N-terminal 20 amino acids of enterovirus A71 VP4 displayed on the hepatitis B core (HBc) virus-like particles (VLP) can induce cross-genotype neutralisation. To investigate if a similar neutralising response against FMDV VP4 could be generated, HBc VLPs displaying the N-terminus of FMDV VP4 were designed. The N-terminal 15 amino acids of FMDV VP4 was inserted into the major immunodominant region. HBc VLPs were also decorated with peptides of the N-terminus of FMDV VP4 attached using a HBc-spike binding tag. Both types of VLPs were used to immunise mice and the resulting serum was investigated for VP4-specific antibodies. The VLP with VP4 inserted into the spike, induced VP4-specific antibodies, however the VLPs with peptides attached to the spikes did not. The VP4-specific antibodies could recognise native FMDV, but virus neutralisation was not demonstrated. This work shows that the HBc VLP presents a useful tool for the presentation of FMDV capsid epitopes.
引用
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页数:11
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