Piwil is essential for gametogenesis in mollusk Chlamys farreri

被引：15

作者：

Ma, Xiaoshi ^{[1
]}

Ji, Aichang ^{[1
]}

Zhang, Zhifeng ^{[1
]}

Yang, Dandan ^{[1
]}

Liang, Shaoshuai ^{[1
]}

Wang, Yuhan ^{[1
]}

Qin, Zhenkui ^{[1
]}

机构：

[1] Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao, Peoples R China

来源：

PEERJ | 2017年 / 5卷

基金：

中国国家自然科学基金;

关键词：

Chlamys farreri; Cf-piwil; Germ cell; Gametogenesis; Testis; Ovary; CELL-DIFFERENTIATION; GENE; PROTEIN; FAMILY; EXPRESSION; ARGONAUTE; SMEDWI-2; BINDING; MEMBER;

D O I：

10.7717/peerj.3412

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Piwi (P-element induced wimpy testis) is an important gene involved in stem cell maintenance and gametogenesis in vertebrates. However, in most invertebrates, especially mollusks, the function of Piwi during gametogenesis remains largely unclear. To further understand the function of Piwi during gametogenesis, full-length cDNA of Piwil from scallop Chlamys farreri (Cf-Piwil) was characterized, which consisted of acid protein. 2,637 by open reading frame encoding an 878-amino acid protein. Cf-Piwil mRNA was mainly localized in the spermatogonia, spermatocytes, oogonia, oocytes of early development and intra-gonadal somatic cells. Additionally, the knockdown of Cf-Piwil by injection of Cf-Piwil-dsRNA (double-stranded RNA) into scallop adductor led to a loss of germ cells in C. farreri gonads. Apoptosis was observed mainly in spermatocytes and oocytes of early development, as well as in a small number of spermatogonia and oogonia. Our findings indicate that Cf-Piwil is essential for gametogenesis in the scallop C farreri.

引用

页数：14

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