Rat N-ERC/Mesothelin as a Marker for In Vivo Screening of Drugs against Pancreas Cancer

被引：4

作者：

Fukamachi, Katsumi ^{[1
]}

Iigo, Masaaki ^{[2
]}

Hagiwara, Yoshiaki ^{[3
]}

Shibata, Koji ^{[1
,4
]}

Futakuchi, Mitsuru ^{[1
]}

Alexander, David B. ^{[2
]}

Hino, Okio ^{[5
]}

Suzui, Masumi ^{[1
]}

Tsuda, Hiroyuki ^{[2
]}

机构：

[1] Nagoya City Univ, Grad Sch Med Sci, Dept Mol Toxicol, Nagoya, Aichi, Japan

[2] Nagoya City Univ, Nanotoxicol Project, Nagoya, Aichi, Japan

[3] Immunobiol Labs, Fujioka, Gunma, Japan

[4] Nagoya Univ, Grad Sch Med, Dept Surg, Div Surg Oncol, Nagoya, Aichi 4648601, Japan

[5] Juntendo Univ, Sch Med, Dept Pathol & Oncol, Tokyo 113, Japan

来源：

PLOS ONE | 2014年 / 9卷 / 10期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

ONCOGENE TRANSGENIC RATS; DUCTAL ADENOCARCINOMAS; MOLECULAR-CLONING; OVARIAN-CARCINOMA; C-ERC/MESOTHELIN; SERIAL ANALYSIS; ELISA SYSTEM; TUMOR-MARKER; EKER RAT; MESOTHELIOMA;

D O I：

10.1371/journal.pone.0111481

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease, which is usually diagnosed in an advanced stage. We have established transgenic rats carrying a mutated K-ras gene controlled by Cre/loxP activation. The animals develop PDA which is histopathologically similar to that in humans. Previously, we reported that serum levels of N-ERC/mesothelin were significantly higher in rats bearing PDA than in controls. In the present study, to determine whether serum levels of N-ERC/mesothelin correlated with tumor size, we measured N-ERC/mesothelin levels in rats bearing PDA. Increased serum levels of N-ERC/mesothelin correlated with increased tumor size. This result indicates an interrelationship between the serum level of N-ERC/mesothelin and tumor size. We next investigated the effect of chemotherapy on serum N-ERC/mesothelin levels. Rat pancreatic cancer cells were implanted subcutaneously into the flank of NOD-SCID mice. In the mice treated with 200 mg/kg gemcitabine, tumor weight and the serum level of N-ERC/mesothelin were significantly decreased compared to controls. These results suggest that serum N-ERC/mesothelin measurements might be useful for monitoring response to therapy.

引用

页数：6

共 29 条

[11]

Hough CD, 2000, CANCER RES, V60, P6281

[12] Secretion of N-ERC/mesothelin and expression of C-ERC/mesothelin in human pancreatic ductal carcinoma [J].

Inami, Koichi ;

Kajino, Kazunori ;

Abe, Masaaki ;

Hagiwara, Yoshiaki ;

Maeda, Masahiro ;

Suyama, Masafumi ;

Watanabe, Sumio ;

Hino, Okio .

ONCOLOGY REPORTS, 2008, 20 (06) :1375-1380

[13] MOLECULAR-CLONING AND EXPRESSION OF MEGAKARYOCYTE POTENTIATING FACTOR CDNA [J].

KOJIMA, T ;

OHEDA, M ;

HATTORI, K ;

TANIGUCHI, Y ;

TAMURA, M ;

OCHI, N ;

YAMAGUCHI, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21984-21990

[14] The N-ERC index is a novel monitoring and prognostic marker for advanced malignant pleural mesothelioma [J].

Mori, Takanori ;

Tajima, Ken ;

Hirama, Michihiro ;

Sato, Tadashi ;

Kido, Kenji ;

Iwakami, Shin-ichiro ;

Sasaki, Shinichi ;

Iwase, Akihiko ;

Shiomi, Kazu ;

Maeda, Masahiro ;

Hino, Okio ;

Takahashi, Kazuhisa .

JOURNAL OF THORACIC DISEASE, 2013, 5 (02) :145-148

[15] Inhibition of Hedgehog Signaling Enhances Delivery of Chemotherapy in a Mouse Model of Pancreatic Cancer [J].

Olive, Kenneth P. ;

Jacobetz, Michael A. ;

Davidson, Christian J. ;

Gopinathan, Aarthi ;

McIntyre, Dominick ;

Honess, Davina ;

Madhu, Basetti ;

Goldgraben, Mae A. ;

Caldwell, Meredith E. ;

Allard, David ;

Frese, Kristopher K. ;

DeNicola, Gina ;

Feig, Christine ;

Combs, Chelsea ;

Winter, Stephen P. ;

Ireland-Zecchini, Heather ;

Reichelt, Stefanie ;

Howat, William J. ;

Chang, Alex ;

Dhara, Mousumi ;

Wang, Lifu ;

Rueckert, Felix ;

Gruetzmann, Robert ;

Pilarsky, Christian ;

Izeradjene, Kamel ;

Hingorani, Sunil R. ;

Huang, Pearl ;

Davies, Susan E. ;

Plunkett, William ;

Egorin, Merrill ;

Hruban, Ralph H. ;

Whitebread, Nigel ;

McGovern, Karen ;

Adams, Julian ;

Iacobuzio-Donahue, Christine ;

Griffiths, John ;

Tuveson, David A. .

SCIENCE, 2009, 324 (5933) :1457-1461

[16] Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma [J].

Onda, Masanori ;

Nagata, Satoshi ;

Ho, Mitchell ;

Bera, Tapan K. ;

Hassan, Raffit ;

Alexander, Richard H. ;

Pastan, Ira .

CLINICAL CANCER RESEARCH, 2006, 12 (14) :4225-4231

[17] Application of mesothelin immunostaining in tumor diagnosis [J].

Ordóñez, NG .

AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 2003, 27 (11) :1418-1428

[18] The utility of serum N-ERC/mesothelin as a biomarker of ovarian carcinoma [J].

Saeki, Harumi ;

Hashizume, Akane ;

Izumi, Hiroshi ;

Suzuki, Fujihiko ;

Ishi, Kazuhisa ;

Nojima, Michio ;

Maeda, Masahiro ;

Hino, Okio .

ONCOLOGY LETTERS, 2012, 4 (04) :637-641

[19] Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma [J].

Scholler, N ;

Fu, N ;

Yang, Y ;

Ye, ZM ;

Goodman, GE ;

Hellström, KE ;

Hellström, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11531-11536

[20] Sensitive and specific new enzyme-linked immunosorbent assay for N-ERC/Mesothelin increases its potential as a useful serum tumor marker for mesothelioma [J].

Shiomi, Kazu ;

Hagiwara, Yoshiaki ;

Sonoue, Kouji ;

Segawa, Tatsuya ;

Miyashita, Kazuya ;

Maeda, Masahiro ;

Izumi, Hiroshi ;

Masuda, Kimihiko ;

Hirabayashi, Masataka ;

Moroboshi, Takao ;

Yoshiyama, Takashi ;

Ishida, Atsuko ;

Natori, Yuji ;

Inoue, Akira ;

Kobayashi, Masashi ;

Sakao, Yukinori ;

Miyamoto, Hideaki ;

Takahashi, Kazuhisa ;

Hino, Okio .

CLINICAL CANCER RESEARCH, 2008, 14 (05) :1431-1437

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